Characterization of novel microsatellite markers in the black-lipped pearl oyster, Pinctada margaritifera (Linnaeus, 175
- PDF / 210,725 Bytes
- 2 Pages / 595.276 x 790.866 pts Page_size
- 69 Downloads / 207 Views
MICROSATELLITE LETTERS
Characterization of novel microsatellite markers in the blacklipped pearl oyster, Pinctada margaritifera (Linnaeus, 1758) Xuzhen Huang • Feng Yu • Jintian Zhu Xuefen Chen • Yan Wang
•
Received: 27 June 2013 / Accepted: 19 July 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract Fourteen microsatellite markers were developed for the black-lipped pearl oyster, Pinctada margaritifera, by mining GenBank microsatellite sequences, redesigning primers. Those markers, characterized in 24 individuals from a wild population, were polymorphic with allele numbers ranging from 3 to 11 per locus. Polymorphism information content ranged from 0.482 to 0.774. Expected and observed heterozygosities ranged from 0.57 to 0.90 and 0.29–0.88 respectively. Four loci showed significant (P \ 0.05 after Bonferroni correction) deviation from Hardy–Weinberg equilibrium, probably because of the presence of null alleles. Most of the microsatellite markers developed here should be useful for genetic breeding and genetic resource conservation of this species. Keywords Microsatellite Pinctada margaritifera Black-lipped pearl oyster Saltwater pearl industry Genetic breeding
The black-lipped pearl oyster, Pinctada margaritifera, is a marine bivalve widely distributed in tropical and subtropical areas of Pacific and Indian Oceans, and mostly inhabited on coral reefs from the low tidal line to tens of Xuzhen Huang and Feng Yu have contributed equally.
Electronic supplementary material The online version of this article (doi:10.1007/s12686-013-0004-8) contains supplementary material, which is available to authorized users. X. Huang F. Yu J. Zhu X. Chen Y. Wang (&) Key Laboratory of Tropical Biological Resources of Ministry of Education, Marine Biology Experiment Teaching Demonstration Center, Ocean College, Hainan University, 58 Renmin Road, Haikou 570228, Hainan, China e-mail: [email protected]
meters in depth. It has been cultivated in several countries (Arnaud-Haond et al. 2003) for the production of black valuable pearl, which could be a new and promising direction of Chinese saltwater pearl industry for this industry is heavily challenged by the low-cost freshwater pearl in China. To develop the aquaculture of black-lipped pearl oyster that bases on a large hatchery production of spats, it requires a number of microsatellite markers to evaluate the genetic diversity of the wild and farmed populations, to assist the selection of founder populations, and to monitor the genetic variation of germplasm resources. Only three (Pmarg7, 11, 45) of ten existing microsatellites developed from French Polynesia P. margaritifera (Herbinger et al. 2006) can amplify consistently in Chinese samples. Here we report the development and characterization of 14 microsatellite makers for this species. The population of P. margaritifera were sampled from inshore reefs of Sanya, China (n = 24). DNA was extracted from ethanol-preserved adductor muscles using standard phenol–chloroform protocol with modified lysis buffer
Data Loading...
