Chemopreventive Effect and HPTLC Fingerprinting Analysis of Jasminum sambac (L.) Ait. Extract Against DLA-Induced Lympho
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Chemopreventive Effect and HPTLC Fingerprinting Analysis of Jasminum sambac (L.) Ait. Extract Against DLA-Induced Lymphoma in Experimental Animals M. Kalaiselvi & R. Narmadha & P. Ragavendran & B. Vidya & D. Gomathi & C. Arul Raj & T. Starlinraj & V. K. Gopalakrishnan & C. Uma & K. Kalaivani
Received: 4 September 2012 / Accepted: 17 December 2012 / Published online: 10 January 2013 # Springer Science+Business Media New York 2013
Abstract The anticancer activity of the ethanolic extract of Jasminum sambac against Dalton’s lymphoma ascites-induced lymphatic cancer in Swiss albino mice was investigated. The anticancer activity of J. sambac was studied against lymphoma using lipid profiles, biochemical parameters, and membrane-bound marker enzymes by standard procedures. A high-performance thin-layer chromatography fingerprinting analysis showed the presence of terpenoids and flavonoids. The levels of cholesterol, triglyceride, VLDL cholesterol, and LDL cholesterol were significantly decreased in tumor-induced mice, while HDL cholesterol showed increased levels compared with those profiles. On treatment with J. sambac, the levels were brought back to near normal. The albumin, creatinine, total protein, urea, and uric acid contents were also approaching normal values. There was s significant increase in the levels of ATPase in group II. These levels were brought back to normal upon plant extract treatment of mice. DNA fragmentation occurred in the tumor-induced group of tissue, and treatment with ethanolic extract reduced the DNA damage caused by lymphoma. Expression of lactate dehydrogenase (LDH) isoenzymes shows an increase in the levels of LDH-4 and LDH-5 in cancer-bearing animals which is brought back to near normal. Histopathological investigation showed normal sections of liver tissues in the treatment group. The results found in mice treated with ethanolic extract 100 mgkg−1 body weight quite promising and were comparable with the standard drug 5-fluorouracil. The statistically processed results support the conclusion that the ethanolic extract of J. sambac flower (100 mgkg−1) possesses a dose-dependent significant anticancer activity against lymphoma.
M. Kalaiselvi : K. Kalaivani (*) Department of Biochemistry, Kongunadu Arts and Science College, Coimbatore 641 029, Tamil Nadu, India e-mail: [email protected] M. Kalaiselvi : R. Narmadha : P. Ragavendran : B. Vidya : D. Gomathi : C. A. Raj : T. Starlinraj : V. K. Gopalakrishnan : C. Uma Department of Biochemistry, Karpagam University, Coimbatore, India
Appl Biochem Biotechnol (2013) 169:1098–1108
1099
Keywords Jasminum sambac . HPTLC fingerprinting . Lymphatic cancer . Terpenoids . Flavonoids . Anticancer
Introduction Cancer is one of the most life-threatening diseases and serious public health problems in both developed and developing countries. It is a group of diseases characterized by the deregulate proliferation of abnormal cells that invade and disrupt surrounding tissues. Due to the toxic and adverse side effects of synthetic drugs, as
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