Chlorothalonil: an effective bacteriostatic agent for bud induction of Acacia auriculiformis under open condition (non-a
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(2019) 15:5 Huang et al. Plant Methods https://doi.org/10.1186/s13007-019-0390-3
Open Access
RESEARCH
Chlorothalonil: an effective bacteriostatic agent for bud induction of Acacia auriculiformis under open condition (non‑axenic) Liejian Huang1*† , Hong Wang1†, Muhammad Qasim Shahid2,3† and Bingshan Zeng1
Abstract Background: Open tissue culture technique could be simplified by using different bacteriostatic agents. There is a great difference in the bacteriostatic effects of different antimicrobial agents on various explants. However, there is no report about the effective bacteriostatic agent for open tissue culture of Acacia auriculiformis. Results: We carried out the bud induction trials under open conditions to screen out an effective antibacterial agent for open tissue culture of A. auriculiformis. The results showed that the suitable type and concentration of bacteriostatic agent was 0.2 g L−1 Chlorothalonil, and the suitable explant type was middle shoot section with leaves (the shoot section with third to fifth axillary bud). The treatment of 0.8 g L−1 Carbendazim for 3 min was the most suitable strategy for explants disinfection, and October was the best time for explants collection. The suitable bud induction medium was 1/8 MS + agar 7 g L−1 + Chlorothalonil 0.2 g L−1 + 6-BA 1.5 mg L−1, and the bud induction rate was 99.54%. Conclusions: Our results revealed that Chlorothalonil is an effective bacteriostatic agent for bud induction of A. auriculiformis under open condition. These results would be very helpful for further establishment of open tissue culture technology for A. auriculiformis. Keywords: Bacteriostatic agent, Chlorothalonil, Bud induction, Open condition, Acacia auriculiformis Background The technique of tissue culture plays an important role in tree breeding, and we can produce a large number of seedlings in limited space using this technique while maintaining excellent characters of the elite plants. However, high temperature and high pressure are usually necessary for sterilization during the process of tissue culture, and seedlings are apt to be polluted. So, the cost of seedling production by tissue culture technique is high. In order to reduce the costs of the seedling production, researchers have tried to find out a new technique *Correspondence: [email protected] † Liejian Huang, Hong Wang and Muhammad Qasim Shahid have contributed equally to this work 1 Research Institute of Tropical Forestry, CAF, 682# Guangshan 1 Road, Guangzhou 510520, Guangdong, China Full list of author information is available at the end of the article
of tissue culture that easy to operate with low pollution rate. Herman pointed out that some plants could be cultured under the non-axenic conditions [1]. According to the Herman’s opinion, some researchers proposed a new tissue culture method that culture the plantlets under open conditions with an appropriate bacteriostatic agent and no need of a strict aseptic technique, and is called as open tissue culture method. During the process of open tissu
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