Clinical Applications of Mass Spectrometry in Biomolecular Analysis
This volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, mul
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Uttam Garg Editor
Clinical Applications of Mass Spectrometry in Biomolecular Analysis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M.Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Clinical Applications of Mass Spectrometry in Biomolecular Analysis Methods and Protocols
Edited by
Uttam Garg Department of Pathology and Laboratory Medicine, Children’s Mercy Hospitals and Clinics, Kansas City, MO, USA
Editor Uttam Garg Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics Kansas City, MO, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3181-1 ISBN 978-1-4939-3182-8 (eBook) DOI 10.1007/978-1-4939-3182-8 Library of Congress Control Number: 2015957377 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Dedication This book is dedicated to my wife Jyotsna and daughters Megha and Mohini who are my inspiration to keep moving forward in life.
Preface Clinical laboratory applications of mass spectrometry are growing rapidly. This has been made possible with the advent of benchtop compact, relatively inexpensive, and userfriendly systems. This versatile technology offers a number of advantages including increased sensitivity and specificity, multiple component analysis, and no need of specialized reagents. In fact the technique is a must for the measurement of many clinically relevant analytes in the fields of drug analysis, endocrinology, and inborn errors of metabolism. This volume Clinical Applications of Mass Spectrometry in Biomolecular Analysis provides stepwise instructions for the analysis
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