Co-infection of a novel fusagravirus and a partitivirus in a Korean isolate of Rosellinia necatrix KACC40168
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SHORT REPORT
Co‑infection of a novel fusagravirus and a partitivirus in a Korean isolate of Rosellinia necatrix KACC40168 Jeesun Chun1 · Dae‑Hyuk Kim1,2,3 Received: 2 June 2020 / Accepted: 1 November 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract We report here the presence of dsRNA mycoviruses in a Korean isolate of Rosellinia necatrix. A multiple band pattern of double-stranded RNA (dsRNA) from R. necatrix suggested mixed mycovirus infection. Next-generation sequencing analysis of purified dsRNAs indicated the presence of two dsRNA mycoviruses related to the members of families “Fusagraviridae” (proposed) and Partitiviridae. The first dsRNA virus revealed that the complete genome sequence was 8868 bp in size and contained two large open reading frames (ORFs 1 and 2), overlapped by 22 bp containing a canonical (− 1) slippery heptanucelotide sequence of UUUAAAC. The deduced amino acid sequence of ORF1 and ORF2 showed highest similarity to the hypothetical protein and RNA-dependent RNA polymerase (RdRp) of Rosellinia necatrix fusagravirus 3 (RnFGV3). Phylogenetic analysis showed that this dsRNA virus clustered with RnFGV3 and other fusagraviruses. Gene organization, sequence similarity, and phylogenetic analysis indicate that this virus seems to belong to a novel species of “Fusagraviridae”, which we have named Rosellinia necatrix fusagravirus 4. The second virus has two dsRNA segments with sizes of 1907 bp and 1918 bp, each of which encoded a single ORF showing highest similarity to the RdRp and capsid protein of known members of Partitiviridae. Evaluation of genome structure, sequence similarity, and phylogeny indicate this to be a new member of the genus Alphapartitivirus in the family Partitiviridae, hereafter designated as Rosellinia necatrix partitivirus 26. This is the first report of the presence of a fusagravirus in an Asian R. necatrix isolate and of its mixed infection with a partitivirus. Keywords Rosellinia necatrix · Mycovirus · Fusagravirus · Partitivirus The fungus Rosellinia necatrix, which belongs to the Xylariaceae family, is a soil-borne fungus that causes serious damage, known as white root rot, to the roots of plants worldwide [1–4]. Although it primarily invades the roots of a wide range of woody and herbaceous plants, more than 400 host
Edited by Seung-Kook Choi. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11262-020-01809-6) contains supplementary material, which is available to authorized users. * Dae‑Hyuk Kim [email protected] 1
Institute for Molecular Biology and Genetics, Jeonbuk National University, Jeonju, Chonbuk, Korea
2
Department of Molecular Biology, Jeonbuk National University, Jeonju, Chonbuk, Korea
3
Department of Bioactive Material Sciences, Jeonbuk National University, Jeonju, Chonbuk, Korea
plant species have been listed by the USDA-ARS (https:// nt.ars-grin.gov/fungaldatabases/). Since the first detection of a mycovirus in this fungus in 2002, a wide variety of
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