Complete nucleotide sequence of a novel partitivirus infecting the plant-pathogenic fungus Phomopsis vexans
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ANNOTATED SEQUENCE RECORD
Complete nucleotide sequence of a novel partitivirus infecting the plant‑pathogenic fungus Phomopsis vexans Chao Jun Zhang1 · Xin Yu Zhou1 · Jie Zhong1 · Jun Guo1 · Xiao Ping Yang2 · Hong Jian Zhu1 Received: 16 August 2020 / Accepted: 5 September 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Here, we report the molecular characterization of a novel partitivirus from Phomopsis vexans strain PvHZ002, a plantpathogenic fungus infecting eggplant. The virus was designated “Phomopsis vexans partitivirus 1” (PvPV1). PvPV1 contains two dsRNA segments, dsRNA1 and dsRNA2, which are 1,662 bp and 1,628 bp long, respectively. Each segment contains a single open reading frame, putatively encoding RNA-dependent RNA polymerase (dsRNA 1) and capsid protein (dsRNA 2). A homology search and phylogenetic analysis showed that PvPV1 clustered with viruses of the genus Deltapartitivirus of the family Partitiviridae. Mycoviruses have been found in almost all major groups of fungi and oomycetes, yeast, mushrooms, and filamentous fungi, since the first report of a mycovirus including Agaricus bisporus infecting in 1962 [1]. Some mycoviruses cause variable effects on the phenotype of their fungal hosts, such as debilitation or changes in virulence [2]. This has been exemplified by the successful use of Cryphonectria hypovirus 1 (CHV1) to control chestnut blight in Europe. Mycoviruses are therefore considered potential biological control agents for fungal crop diseases [2–5].
Handling Editor: Ioly Kotta-Loizou. Chao Jun Zhang and Xin Yu Zhou contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04835-9) contains supplementary material, which is available to authorized users. * Xiao Ping Yang [email protected] * Hong Jian Zhu [email protected] 1
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda road 1, Furong district, Changsha 410128, China
Hunan Biological and Electromechanical Polytechnic, Longping Hi‑Tech Park, Furong district, Changsha 410127, China
2
Viruses in the family Partitiviridae are divided into five genera: Alphapartitivirus, Betapartitivirus, Gammapartitivirus, Deltapartitivirus and Cryspovirus [6]. The majority of viruses in this family have genomes composed of two dsRNA segments, ranging in size from 1.4 to 2.4 kbp, encoding an RNA-dependent RNA polymerase (RdRP) and a capsid protein (CP), respectively [6–9]. Virus particles are isometric, non-enveloped, and approximately 30-35 nm in diameter [10]. Members of each genus of the family Partitiviridae have a characteristic host viruses in the genera Alphapartitivirus and Betapartitivirus mainly infect plants or fungi, those in the genus Gammapartitivirus infect fungi, and those in the genera Deltapartitivirus and Cryspovirus infect plants and protozoa, respectively [11, 12]. Some mycoviruses have been shown to be phylogenetically related to viru
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