Colorimetric and fluorescent dual-identification of glutathione based on its inhibition on the 3D ball-flower shaped Cu-
- PDF / 2,795,048 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 10 Downloads / 141 Views
ORIGINAL PAPER
Colorimetric and fluorescent dual-identification of glutathione based on its inhibition on the 3D ball-flower shaped Cu-hemin-MOF’s peroxidase-like activity Xiaolong Chen 1 & Xianfeng Wang 1 & Gaihua Cao 1 & Yawen Wu 1 & Huibo Luo 2 & Zhong Ji 1 & Caihong Shen 1,3 & Danqun Huo 1,2 & Changjun Hou 1,4 Received: 9 May 2020 / Accepted: 21 September 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Hemin as the organic linker ligand and Cu (II) as the metal center were applied to prepare a copper-metal-organic framework (Cuhemin-MOF) via one-step hydrothermal method. Characterization using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD) demonstrate that the acquired Cu-hemin-MOF possesses the appearance of 3D ball-flower shape with the existence of C, N, O, Fe, and Cu on the surface. Further study found that this 3D ball-flower shaped Cu-hemin-MOF exhibits peroxidase-like activity, which can catalyze the peroxidase substrate of o-phenylenediamine (OPD) to generate 2,3-diaminophenazine (DAP) in the presence of H2O2. DAP has a yellow color and also emits a strong fluorescence when excited by ultraviolet light. Interestingly, Cu-hemin-MOF’s peroxidase-like activity can be strongly inhibited by glutathione (GSH). In view of this, a dual readout (fluorescence and colorimetry) was proposed to detect GSH for the first time. Under optimal conditions, the proposed method exhibits good linear relationship between the signal response (fluorescence and colorimetry) and the concentration of GSH, and low limits of detection (LOD) of 2.3 and 26.6 nM, respectively. Keywords Glutathione . Metal-organic frameworks . O-Phenylenediamine . Fluorescence . Colorimetry
Introduction Glutathione (GSH) plays a crucial role in the processes of physiological and biological such as metabolism, detoxification, gene regulation, and maintenance of the intracellular redox
homeostasis [1–3]. Normal level of GSH in individual physiological fluid is one of the signs of body health. But numerous clinical diseases such as Alzheimer’s disease [4, 5], liver disease [6], oxidative stress [7], psychiatric disorders [8], defects in enzymes of the γ-glutamyl cycle [9], and HIV [10, 11] can lead to
Xiaolong Chen and Xianfeng Wang contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04565-4) contains supplementary material, which is available to authorized users. * Caihong Shen [email protected]
2
Liquor Making Biology Technology and Application of Key Laboratory of Sichuan Province, College of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, People’s Republic of China
3
National Engineering Research Center of Solid-State Brewing, Luzhou Laojiao Group Co. Ltd., Luzhou 646000, People’s Republic of China
4
Chongqing Key Laboratory of Bio-perception & Intelligent Information Processing, School of M
Data Loading...