A dual-modal colorimetric and photothermal assay for glutathione based on MnO 2 nanosheets synthesized with eco-friendly
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RESEARCH PAPER
A dual-modal colorimetric and photothermal assay for glutathione based on MnO2 nanosheets synthesized with eco-friendly materials Dandan Liu 1 & Qingqing Tu 1 & Ying Han 2 & Xiaoying Wang 1,3 & Qing Kang 1 & Pengcheng Wang 1
&
Wenjuan Guo 1
Received: 28 July 2020 / Revised: 16 September 2020 / Accepted: 30 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract We developed a dual-modal colorimetric and photothermal assay for glutathione (GSH) using MnO2 nanosheets prepared with environmentally friendly materials. The nanosheets were synthesized by using ascorbic acid present abundantly in lemon and orange juices to reduce KMnO4. The as-prepared MnO2 nanosheets display oxidase-like activity and can catalyze the oxidation reaction of 3,3′,5,5′-tetramethylbenzidine (TMB), yielding a blue oxidative product (oxTMB) that exhibits a UV–Vis absorption peak at 652 nm. In the presence of GSH, the MnO2 nanosheets are reduced and decomposed, resulting in a decrease in the peak intensity. The colorimetric assay offers a wide dynamic range (0.1–100 μM) and a detection limit of 100 nM. The MnO2 nanosheets are also efficient in converting photoenergy to thermal energy, with a photothermal conversion efficiency of 23.3%. The temperature change, after near-infrared (NIR) irradiation at 808 nm, can be easily measured by an inexpensive pen-type thermometer. This effect can also be used for GSH quantification and expands the GSH concentration detection to the range from 6.0 to 200 μM. The viability of our dual-modal assay for clinical applications was demonstrated with successful analyses of GSH in human serum samples. Keywords MnO2 nanosheets . Dual-modal assay . Colorimetry . Photothermal analysis . GSH
Introduction Glutathione (GSH), a vital non-protein mercaptan, is the most abundant antioxidant found in eukaryotic cells [1, 2]. It acts as a free radical scavenger and detoxifying agent in multiple biological processes, which include, but are not limited to, cell redox homeostasis, signal transduction, and Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00216-020-02982-1) contains supplementary material, which is available to authorized users. * Pengcheng Wang [email protected] * Wenjuan Guo [email protected] 1
Institute of Surface Analysis and Chemical Biology, University of Jinan, Jinan 250022, Shandong, China
2
Department of Oncology and Radiotherapy, Shandong Provincial Qianfoshan Hospital, The First Hospital Affiliated with Shandong First Medical University, Jinan 250014, Shandong, China
3
State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Jinan 250353, Shandong, China
gene regulation [1, 2]. Multiple lines of evidence indicate that GSH deficiency and/or imbalance is associated with a wide range of diseases, such as neurodegenerative disorders, diabetes, and cancers [2, 3]. Consequently, developing reliable and simple detection of GSH has attracted considerable attention in
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