Colorimetric Detection of Uric Acid with High Sensitivity Using Cu 2 O@Ag Nanocomposites
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ORIGINAL ARTICLE
Colorimetric Detection of Uric Acid with High Sensitivity Using Cu2O@ Ag Nanocomposites Xue Wang1 · Jiajun Lu1 · Xiaomin Tang2 · Ping Qiu1 Received: 27 November 2019 / Accepted: 7 February 2020 © The Tunisian Chemical Society and Springer Nature Switzerland AG 2020
Abstract In this paper, we built the Cu2O@Ag nanocomposites-catalyzed 3,3′,5,5′-tetramethylbenzidine (TMB)–H2O2 system for sensitive, rapid, and low-cost colorimetric sensing of uric acid (UA). Firstly, Ag nanotriangles were synthesized by dynamic method, and then Cu2O was encapsulated on the nanoflakes to synthesize Cu2O@Ag nanocomposites. Characterization of the structure and morphology of nanocomposites material were characterized by transmission electron microscopy, X-ray energy-dispersive spectroscopy, X-ray diffraction and X-ray photoelectron spectroscopy. C u2O@Ag nanocomposites could catalyze the oxidation of TMB by H2O2 (which is produced by enzymatic oxidation of UA), and the color changes from colorless to blue can be observed directly with the naked eyes or can be detected by UV–Vis spectrophotometer. The colorimetric sensor provided a linear absorbance response in the 0.5–100 μM UA concentration range with a 0.4 μM detection limit (S/N = 3). The recovery of UA in human serum by the proposed sensor is over 95.7%. Thus, these results imply that the UA sensor provides an effective tool in fast clinical analysis of gout without the need for expensive and advanced equipment. Keywords Uric acid · Uricase · Cu2O@Ag nanocomposites · TMB · Serum samples
1 Introduction Uric acid (2.6.8-trihydroxypurine, UA) is an important biomolecule in human body, and is often used as a diagnostic indicator [1]. The normal concentration of UA in human serum ranges from 130 to 460 μM [2]. Hyperuricemia is caused by increased UA secretion, which has been identified as a major contributor to gout [3]. However, hyperuricemia is often accompanied by hypertension, diabetes, kidney disease, obesity and coronary heart disease [4]. Therefore, the focus of the research is to develop a rapid sensor for monitoring UA levels in human body fluids for clinical examination and disease diagnosis. A number of methods have been established for the determination of UA, such as electrochemical methods [5], chromatography [6], capillary electrophoresis [7], fluorescence methods [8], paper based technique [9] colorimetry [10], * Ping Qiu [email protected] 1
Department of Chemistry, Nanchang University, Nanchang 330031, China
The Fourth Affiliated Hospital of Nanchang University, Nanchang 330003, China
2
and molecular spectroscopy [11]. Since the electrochemical methods and chromatography are time-consuming, expensive equipment, and inconvenient and easily interfered, these methods were often limited in use [12, 13]. Among these methods, the colorimetric method has raised widespread interest because of its easy identification of color changes, rapidity, convenience, high sensitivity, and low consumption [14–16]. Therefore, colorimetry has become an i
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