Comparative Characteristics of the Spleen White Pulp in Experimental Models of Sepsis Caused by Different Strains of Pse
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eristics of the Spleen White Pulp in Experimental Models of Sepsis Caused by Different Strains of Pseudomonas aeruginosa M. N. Cherkasova, V. G. Zhukhovitskii, and T. G. Borovaya
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 7, pp. 57-60, July, 2020 Original article submitted March 4, 2020 We describe reactive changes in the spleen white pulp in male C57BL/6 mice with experimental sepsis induced by intraperitoneal administration of Pseudomonas aeruginosa 1840 (Pa1840) with exotoxin U gene or Pseudomonas aeruginosa 1623 (Pa1623) with exotoxin S gene. Histological analysis and morphometry revealed hypoplasia of the spleen white pulp in mice with sepsis induced by Pa1840, while sepsis caused by Pa1623 was associated with its hyperplasia; with apoptosis of white pulp cells was observed in both cases. The results attest to ambiguous nature of the reactive changes in the white pulp of the spleen in experimental sepsis models initiated by Pa1840 and Pa1623 stains. Key Words: sepsis; Pseudomonas aeruginosa; spleen; white pulp
The idea of the pathogenesis of sepsis needs to be substantially clarified, in particular, its regularities and details that depend on the type and strain of the pathogens. An important source of information is experimental modeling [1,5], for instance, infection of animals with different strains of microorganisms cultured from the primary foci of infection and blood of septic patients. Analysis of histological changes in organs of infected animals, in particular, organs of the immune system, will contribute to not only the formation of a more holistic and objective knowledge of sepsis as a systemic inflammatory process, but also elucidation of its pathogenetic details and course depending on the characteristics of the pathogen. Our aim was comparative analysis of the reactive changes in the white pulp of the spleen in mice with experimental sepsis induced by Pseudomonas aeruginosa 1840 and 1623 strains. Laboratory for Indication and Structural Analysis of Microorganisms, N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia. Address for correspondence: [email protected]. M. N. Cherkasova
MATERIALS AND METHODS In mature male C57BL/6 mice weighing 18-20 g, sepsis was modeled using two strains of Pseudomonas aeruginosa — 1840 and 1623 (Pa1840 and Pa1623), isolated from the burn wound of a patient of a burn unit and from bronchial lavage fluid of a patient on artificial lung ventilation in an intensive care unit, respectively. All manipulations with animals were performed in accordance with the International Recommendations for Biomedical Research Using Animals (1989). The animals were randomized into 2 experimental and one control groups. Control animals (n=3) were intraperitoneally injected with 0.5 ml sterile saline. Experimental group 1 included 4 subgroups (6 mice each) that received single intraperitoneal injection of a 24-h broth culture of Pa1840 in a volume of 0.5 ml in d
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