Comparative Genomic Study of Polar Lichen-Associated Hymenobacter sp. PAMC 26554 and Hymenobacter sp. PAMC 26628 Reveals
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Comparative Genomic Study of Polar Lichen‑Associated Hymenobacter sp. PAMC 26554 and Hymenobacter sp. PAMC 26628 Reveals the Presence of Polysaccharide‑Degrading Ability Based on Habitat Nisha Ghimire1 · So‑Ra Han1 · Byeollee Kim1 · Hyun Park2 · Jun Hyuck Lee3,4 · Tae‑Jin Oh1,5,6 Received: 3 February 2020 / Accepted: 7 July 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The genus Hymenobacter is classified in the family Hymenobacteraceae under the phylum Bacteroidetes. They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions. The polar regions have attracted intense research interest for the discovery of novel microorganisms and their functions. Analysis of the polysaccharide utilization-related carbohydrate-active enzyme among the two lichenassociated polar organisms Hymenobacter sp. PAMC 26554 and Hymenobacter sp. PAMC 26628 was performed, along with its comparison with the complete genome of the same genus available in the NCBI database. The study was conducted relying on the AZCL screening data for the two polar lichen-associated species. While comparing with eight other complete genomes, differences in polysaccharide preferences based on the isolation environment and biosample source were discovered. All the species showed almost similar percentage of cellulose synthesis and degradation genes. However, the polar lichen-associated microorganism was found to have a high percentage of hemicellulose degradation genes, and less starch and laminarin degradation. The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
Introduction
* Jun Hyuck Lee [email protected] * Tae‑Jin Oh [email protected] 1
Department of Life Science and Biochemical Engineering, Graduate School, SunMoon University, Asan 31460, Korea
2
Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea
3
Unit of Research for Practical Application, Korea Polar Research Institute, Incheon 21990, Korea
4
Department of Polar Sciences, University of Science and Technology, Incheon 21990, Korea
5
Genome-Based BioIT Convergence Institute, Asan 31460, Korea
6
Department of Pharmaceutical Engineering and Biotechnology, SunMoon University, Asan 31460, Korea
Up to 80% of the Earth’s surface is covered by a cold ecosystem [1]. Recently, polar regions have been enthusiastically investigated to explore new possibilities for microbiomes [2]. Cold-active microorganisms, designated as psychrophilic microorganism, have colonized environments such as high mountains, polar regions, and deep sea. Although these extreme habitats are very challenging for the organism, they have developed cold adaptation strategies for their survival. The features of the microorgani
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