Hymenobacter busanensis sp. nov., radiation-resistant species isolated from soil in South Korea
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ORIGINAL PAPER
Hymenobacter busanensis sp. nov., radiation‑resistant species isolated from soil in South Korea Sang Eun Lee1 · Leonid N. Ten2 · Yuna Park1 · Soohyun Maeng1 · Jing Zhang3 · Min‑Kyu Kim3 · In‑Tae Cha4 · Ki‑Eun Lee4 · Byoung‑Hee Lee4 · Hee ‑ Young Jung2,5 · Myung Kyum Kim1 Received: 8 July 2020 / Revised: 3 September 2020 / Accepted: 1 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Two bacterial strains designated as MA3T and BT182 were isolated from a soil sample in South Korea. Cells of the two strains were Gram-stain-negative, non-motile, rod-shaped and formed red colonies on R2A agar at 25 °C. The 16S rRNA genes of the two strains shared a sequence similarity of 99.8%. Both strains shared the highest 16S rRNA gene similarity of 96.8% with Hymenobacter edaphi NLT, followed by Hymenobacter paludis KBP-30T (96.3%), Hymenobacter coalescens WW84T (96.3%) and Hymenobacter gummosus ANT-18T (96.3%). Growth was observed at 15–37 °C (optimum 30 °C), pH 6–8 (optimum pH 7) and in the presence up to 1% NaCl. The genome size of strains MA3T and BT182 is 4.9 Mb and 4.8 Mb, respectively. The genomic G + C content of both strains is 62.0 mol%. The main polar lipid of the strains was phosphatidylethanolamine, the only respiratory quinone detected was menaquinone-7 and the major fatty acids were anteiso-C15:0, iso-C15:0, summed feature 4 (iso-C17:1 I/anteiso-C17:1 B) and summed feature 3 (C16:1 ω6c/C16:1 ω7c), supporting the affiliation of these strains with the genus Hymenobacter. Based on the phylogenetic, genotypic, phenotypic and chemotaxonomic data, strains MA3T and BT182 represent a novel species of the genus Hymenobacter, for which the name Hymenobacter busanensis is proposed. The type strain is MA3T (= KCTC 72631T = NBRC 114193T). Keywords Hymenobacter · Hymenobacteraceae · Soil bacteria
Communicated by Erko stackebrandt. Sang Eun Lee and Leonid N. Ten contributed equally to this work. The GenBank accession numbers for the 16S rRNA gene sequences of strain MA3T and strain BT182 are MN396447 and MN555344, respectively. The whole-genome sequences of strain MA3T and strain BT182 have been deposited into DDBJ/EMBL/ GenBank under the accession numbers VTWU00000000 and CP047647, respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02080-x) contains supplementary material, which is available to authorized users.
Introduction The genus Hymenobacter, a member of the family Hymenobacteraceae (Munoz et al. 2016) of the phylum Bacteroidetes, was first described by Hirsch et al. (1998) and was later emended by Buczolits et al. (2006) and Han et al. (2014). At the time of writing, the genus comprised 83 species with validly published names (https://lpsn.dsmz.de/ search ?word=hymeno bacte r). Hymenobacter species occupy a wide range of ecological niches (Hirsch et al. 1998; Buczolits et al. 2006; Chen et al. 2016, 2017; Kang et al. 2016,
* Hee ‑ Young Jung [email protected]; [email protected]
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