Comparative genomics of Lactobacillus species as bee symbionts and description of Lactobacillus bombintestini sp. nov.,
- PDF / 790,603 Bytes
- 11 Pages / 612 x 792 pts (letter) Page_size
- 71 Downloads / 287 Views
eISSN 1976-3794 pISSN 1225-8873
Comparative genomics of Lactobacillus species as bee symbionts and description of Lactobacillus bombintestini sp. nov., isolated from the gut of Bombus ignitus§ Jun Heo, Soo-Jin Kim, Jeong-Seon Kim, Seung-Beom Hong, and Soon-Wo Kwon* Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Jeollabuk-do 55365, Republic of Korea (Received Dec 13, 2019 / Revised Feb 20, 2020 / Accepted Feb 21, 2020)
The Lactobacillus genus is widely used for fermentation of plant materials and dairy products. These species are typically found in highly specialized environments, with the bee gut serving as one of the niche locations in which Lactobacillus is detected. Lactobacillus species isolated from the bee gut and bee-related habitats were phylogenetically classified into three distinct groups, Lactobacillus kunkeei, Firm-4, and Firm-5. The L. kunkeei group was clearly differentiated from other members of the Lactobacillus buchneri group isolated from non-bee habitats. In comparison with non-bee members of the L. buchneri group, three bee-symbiotic Lactobacillus groups had a small-sized genome with low G + C content and showed a sharp reduction in the number of genes involved in energy production, carbohydrate transport and metabolism, and amino acid transport and metabolism. In addition, all three groups lacked the mutY gene, which encodes A/G-specific adenine glycosylase. The phylogenetic dendrogram based on the presence or absence of 1,199 functional genes indicated that these bee-symbiotic groups experienced convergent evolution. The occurrence of convergent evolution is thought to stem from the three bee-symbiotic groups sharing a similar habitat, i.e., the bee gut. The causative factor underlying genomic reduction was postulated to be mutY, which was absent in all three groups. Here, a novel strain, BHWM-4T, isolated from the gut of Bombus ignites was studied using polyphasic taxonomy and classified as a new member of the L. kunkeei group. The strain was Gram-positive, facultative anaerobic, and rod-shaped. The 16S ribosomal RNA gene sequence and genome analysis revealed that strain BHWM-4T was clustered into the L. kunkeei group, forming a compact cluster with L. kunkeei and Lactobacillus apinorum. Biochemical, chemotaxonomic, and genotypic data of strain BHWM-4T supports the proposal of a novel species, Lactobacillus bombintestini sp. nov., whose type strain is BHWM-4T (= KACC *For correspondence. E-mail: [email protected]; Tel.: +82-63-2383024; Fax: +82-63-238-3845 § Supplemental material for this article may be found at http://www.springerlink.com/content/120956. Copyright G2020, The Microbiological Society of Korea
19317T = NBRC 113067T). Keywords: Lactobacillus bombintestini, Bombus ignitus, bee gut, mutY Introduction The genus Lactobacillus has been widely used for fermentation of plant materials, dairy products, and meat (Canchaya et al., 2006). It is comprised of more than 230 species, with Lactobacillus delbrueckii as th
Data Loading...
