Thiosulfativibrio zosterae gen. nov., sp. nov., and Thiosulfatimonas sediminis gen. nov., sp. nov.
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ORIGINAL PAPER
Thiosulfativibrio zosterae gen. nov., sp. nov., and Thiosulfatimonas sediminis gen. nov., sp. nov. Jun Mochizuki1,2 · Hisaya Kojima1 · Manabu Fukui1 Received: 8 September 2020 / Revised: 30 September 2020 / Accepted: 10 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Aerobic, Gram-stain-negative, obligately chemolithoautotrophic thiosulfate-oxidizing bacteria, strains A kT22T and a ks77T T T were isolated from a brackish lake in Japan. Strains AkT22 and aks77 were isolated from samples of eelgrass and sediment, respectively. Growth on sulfide, tetrathionate, elemental sulfur, and organic substrates was not observed for both strains. Growth of the strains was observed at 5 °C or higher temperature, with optimum growth at 22 °C. Strain AkT22T grew at a pH range of 5.8–8.0, with optimum growth at pH 6.7–7.8. Strain aks77T grew at a pH range of 5.8–8.5, with optimum growth at pH 7.0–7.9. Major cellular fatty acids (> 10% of total) of strain AkT22T were C 16:1, C18:1, and C 16:0. The sole respiratory quinone was ubiquinone-8 in both strains. The genome of strain A kT22T consisted of a circular chromosome, with size of approximately 2.6 Mbp and G + C content of 43.2%. Those values of the genome of strain aks77T were ca. 2.7 Mbp and 45.5%, respectively. Among cultured bacteria, Thiomicrorhabdus aquaedulcis HaS4T showed the highest sequence identities of the 16S rRNA gene, to strains AkT22T (94%) and aks77T (95%). On the basis of these results, Thiosulfativibrio zosterae gen. nov., sp. nov. and Thiosulfatimonas sediminis gen. nov., sp. nov. are proposed, with type strains of AkT22T (= BCRC 81184T = NBRC 114012T = DSM 109948T) and aks77T (= BCRC 81183T = NBRC 114013T), respectively. Keywords Sulfur-oxidizing bacteria · Chemolithoautotroph · Thiomicrorhabdus · Brackish lake · Novel genus
Introduction The genus Thiomicrorhabdus in the family Piscirickettsiaceae was originally established with four species, Thiomicrorhabdus frisia, Thiomicrorhabdus chilensis, Thiomicrorhabdus arctica and Thiomicrorhabdus psychrophila (Boden Communicated by Erko Stackebrandt. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains AkT22T and aks77T are LC510548 and LC510549, respectively. The numbers for their complete genomes are AP021888 (AkT22T) and AP021889 (aks77T). Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02090-9) contains supplementary material, which is available to authorized users.
et al. 2017a). Immediately after that, Thiomicrorhabdus hydrogeniphila was added to the genus as a result of reclassification (Boden et al. 2017b). These five species were originally described as Thiomicrospira species (Brinkhoff et al. 1999a, b; Knittel et al. 2005; Watsuji et al. 2016). In the genus Thiomicrorhabdus, the first non-marine species was described as Thiomicrorhabdus aquaedulcis (Kojima & Fuki 2019), and the most recently described species is Thiomicrorhabdus indic
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