Integrative analysis of transcriptomic and proteomic changes related to male sterility in Tagetes erecta
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RESEARCH ARTICLE
Integrative analysis of transcriptomic and proteomic changes related to male sterility in Tagetes erecta Nan Tang1,2,3 • Wei Liu4 • Wuhua Zhang1 • Daocheng Tang1
Received: 7 May 2020 / Revised: 24 August 2020 / Accepted: 18 September 2020 Ó Prof. H.S. Srivastava Foundation for Science and Society 2020
Abstract Male sterile and male fertile two-type lines are important in heterosis utilization and breeding in Tagetes erecta, but the genes and pathways involved in male sterility are poorly understood. To explore these topics, transcriptome data (by RNA-seq) and proteome data (by iTRAQ) were gathered from flower buds of the male sterile line ‘MS2-2’ and male fertile line ‘MF2-2’ and integrated for a better understanding of the underlying molecular mechanisms of male sterility in T. erecta. The RNA-seq procedure generated 285,139,740 clean reads and 63359 unigenes and 6640 differentially expressed genes (DEGs) were identified, of which 4136 were downregulated and 2504 were upregulated in ‘MS2-2’. DEGs related to flower development, pollen development, pollen wall assembly, endogenous hormones and transcription factors were identified. The iTRAQ analysis identified 3950 proteins in total; 789 were differentially expressed proteins (381 Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12298-020-00886-z) contains supplementary material, which is available to authorized users. & Daocheng Tang [email protected] 1
Plateau Flower Research Centre, Department of Agriculture and Husbandry, Qinghai University, Xining 810016, People’s Republic of China
2
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, People’s Republic of China
3
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, People’s Republic of China
4
College of Agriculture, Henan University of Science and Technology, Luoyang 471003, People’s Republic of China
upregulated, 408 downregulated), which were mainly annotated to the Ribosome, Carbon metabolism and Biosynthesis of amino acids pathways. An association analysis revealed strong correlation (r Pearson = 0.6019) between the transcriptomic and proteomic data, and 256 and 34 proteins showed the same and opposite expression patterns with regard to their transcripts, respectively. Pathways such as photosynthesis, fatty acid biosynthesis and phenylpropanoid biosynthesis which influence tapetum and pollen development in male sterile plants, were significantly enriched at the transcript and protein levels. Most genes involved in these pathways were downregulated in ‘MS2-2’. The low expression of these genes or functional loss of proteins could be associated with flower development, pollen development and related to changes in fertility in T. erecta. This study provided transcriptomic and proteomic information for T. erecta that could illuminate the mechanism of male sterility. Keywords Marigold Transcriptome
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