Comparative proteomic study on fem-1b in female and male gonads in Hyriopsis cumingii
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Comparative proteomic study on fem-1b in female and male gonads in Hyriopsis cumingii Ya-Yu Wang 1,2,3 & Sheng-Hua Duan 1,2,3 & Sai-Sai Dong 1,2,3 & Xiao-Yu Cui 1,2,3 & Gui-Ling Wang 1,2,3 & Jia-Le Li 1,2,3 Received: 13 May 2020 / Accepted: 15 September 2020/ # Springer Nature Switzerland AG 2020
Abstract
Sex regulation of bivalves is a complex process, and there has also been little research on their sex-determining genes. To better understand the sex determination/differentiation of bivalves, we analyzed the gonadal proteomics of Hyriopsis cumingii. We identified 673 peptides and 76 proteins. The 35 differentially expressed proteins identified included 19 upregulated and 16 downregulated proteins. FEM-1B protein associated with sex determination showed female-specific expression; the function of fem-1b gene was explored. The full-length cDNA of fem-1b cloned by RACE was 2105 bp. Quantitative PCR (qPCR) was performed on male and female tissues and early developmental stages; the results showed that fem-1b was expressed in both male and female tissues, and its expression level in gonads was significantly higher in females than in males (P < 0.01). In early embryonic development, the fem-1b mRNA expression increased significantly on the third day after fertilization (cleavage stage). Results of in situ hybridization showed a strong signal on oocyte membranes and no signal in male gonads. Our results indicate that fem-1b plays a certain role in sex determination and oocyte development of H. cumingii. This study provides valuable resources for understanding the mechanism and pathway of sex determination/differentiation of H. cumingii. Keywords Label-free . Fem-1b . Sex determination/differentiation . Hyriopsis cumingii
Introduction The term proteome refers to all the proteins expressed by a cell, a tissue, or an organism. Unlike traditional protein research, proteomics can identify the expression level, modification This article is part of the Topical Collection on Epigentic in Aquaculture Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10499-02000605-1) contains supplementary material, which is available to authorized users.
* Gui-Ling Wang [email protected] Extended author information available on the last page of the article
Aquaculture International
level, and interaction of thousands of proteins at one time on a large scale, thus revealing the role of proteins in life processes (Wilkins et al. 1996). Proteomics has been widely used to understand the complex processes of sex differentiation and development in aquatic animals. For instance, comparative proteomics was used to compare the proteins in mature and immature spermatozoa of Cranoglanis bouderius and to screen the proteins (PLCγ, PAK, Raf, and GSK-3) that play an important role in spermatogenesis (Chen et al. 2016). A study of the dynamic changes in the protein spectrum of zebrafish oocytes during maturation showed that proteins such as vitellogenin (Vtg3), elongation factor 1-α and 17 β-hydroxysteroi
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