Comparative Study of the Antimicrobial Effect of Different Antibiotics Mixed with CuO, MgO and ZnO Nanoparticles in Stap
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Comparative Study of the Antimicrobial Effect of Different Antibiotics Mixed with CuO, MgO and ZnO Nanoparticles in Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli cultures
Raúl Alenó1, Anthony López Collazo1, Eulalia Medina 1, Lourdes Díaz Figueroa 1 , José I. Ramírez1 and Edmy J. Ferrer Torres1*. 1
Department of Science and Technology, Inter American University of Puerto Rico, Ponce Campus, Mercedita, P.R. 00715. *Research Director, [email protected]
ABSTRACT Due to the rapid advance of the emergence of resistant microorganisms to different antibiotics, there is a need to create new antimicrobial agents. It is possible that Nanotechnology has a great impact in this area since the nanoparticles can improve the antimicrobial effect of the antibiotics. In this study we used three different metal oxides nanoparticles, the MgO, ZnO and CuO. These nanoparticles were selected because their interactions leading to cell death and their optical properties. The aim of this study is to develop new methods that are more effective against resistance bacteria, developing antibacterial agents using different nanoparticles against Escherichia coli (ATCC 10536), Pseudomonas aeruginosa (ATCC 10145), and Staphylococcus aureus (ATCC BAA-1026). This study was conducted to evaluate the antibacterial effects of a combination of nanoparticles together with different concentrations of three antibiotics, Gentamicin, Cephalexin and Co-Trimoxazole. The results showed that some nanoparticles are effective to inhibit growth in these microorganisms by increasing the effectiveness of the antibiotic. Therefore, the present study indicates that the combination of the nanoparticles with antibiotics may be applicable as a new antimicrobial agent.
INTRODUCTION
The use of nanotechnology for the treatment of bacterial infections is an innovative strategy to enhance the effect of antibiotics. Recovery from bacterial infections is challenged due to the indiscriminate use and abuse of antibiotics [1]. Therefore, nanoparticles can be used to develop new treatments against resistant bacterial infections increasing the potential of antibiotics. This study presents antimicrobial activity of enhanced antibiotics mixed with CuO, MgO and ZnO nanoparticles. There is evidence indicating the antimicrobial effectiveness of ZnO, CuO and MgO [2,3,4]. Our goal is to develop more effective treatments against resistant bacterial infections combing antibiotics and nanoparticles. The antibiotics chosen to perform this study were Gentamicin, Cephalexin, and Co-Trimoxazole. Nanoparticles of MgO, CuO and ZnO were mixed with antibiotics, at different proportions and concentrations to determine their effectiveness.
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EXPERIMENT
Nanoparticles were synthesized through the modification of reported methods in the literature [5,6]. CuO nanoparticles were synthesized dissolving 0.2497 g of copper (II) sulfate pentahydrate salt (CuSO4.5H2O) 0.01 M in 20 ml of PEG 0.02 M. The amount of ascorbic acid 0.02 M, added by titration, was 18ml. 500 μl of NaOH
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