Comparative transcriptome combined with morpho-physiological analyses revealed candidate genes potentially for different
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ORIGINAL ARTICLE
Comparative transcriptome combined with morpho‑physiological analyses revealed candidate genes potentially for differential cold tolerance in two contrasting apricot (Prunus armeniaca L.) cultivars Dan Yu1 · Xiaojuan Liu1 · Yifan Cui1 · Quanxin Bi1 · Yang Zhao1 · Dongxing Li1 · Haiyan Yu1 · Libing Wang1 Received: 5 December 2019 / Accepted: 18 May 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message We performed a comparative transcriptome combined with morpho-physiological analyses between two differential cold tolerance in two contrasting apricot cultivars. The revealed candidate genes potentially were identified by differential expression analysis. Apricot (Prunus armeniaca L.) is a major (non-wood product) forest tree species that is adversely affected by spring frost. However, the mechanisms regulating gene expression in response to frost under natural conditions remains relatively uncharacterized. In this study, we analyzed the phenotypic, anatomical, physiological and molecular characteristics of pistils from two apricot cultivars that differ regarding cold tolerance under spring frost. A total of 217,142,216 and 218,148,439 clean reads were obtained for the cold-tolerant (‘Weixuan 1’) and the cold-sensitive (‘Longwangmao’) sample libraries, respectively. Moreover, 57,689 unigenes (≥ 200 bp long) were identified, with an N50 of 2468 bp and a mean length of 1038.08 bp. Furthermore, 1116 up-regulated and 428 down-regulated differentially expressed genes (DEGs) were identified in the two apricot cultivars. Thirteen DEGs related to Ca2+ signaling, four DEGs involved in MAPK cascades, and thirty DEGs encoding transcription factors were affected by spring frost. The most significant transcriptome changes induced by spring frost were related to carbohydrate metabolism, amino acid metabolism, lipid metabolism, secondary metabolites, plant hormone signal transduction, and terpenoid metabolism. Notably, bHLH75, GDSL esterase/lipase EXL3, GDSL esterase/lipase LTL1, and peroxidase-66 were more highly expressed in ‘Weixuan 1’ than in ‘Longwangmao’ under 6:00, suggesting these genes may contribute to the cold tolerance of ‘Weixuan 1’. The reliability of our RNA sequencing results was confirmed by quantitative real-time PCR. The data generated in this study may form the basis of future studies on the mechanisms underlying the cold tolerance of apricot under natural field conditions. Keywords Apricot · Spring frost · Transcriptome · Differentially expressed genes · RT-qPCR
Introduction Communicated by Francisco M. Cánovas. Dan Yu and Xiaojuan Liu have contributed equally to this article. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00468-020-01991-y) contains supplementary material, which is available to authorized users. * Libing Wang [email protected] 1
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, National Forestry and Grassland Administration, Research
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