Effects of Exogenous Soybean Isoflavones on Physiological Parameters of Achyranthes bidentata Blume Seedlings under UV-B
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Effects of Exogenous Soybean Isoflavones on Physiological Parameters of Achyranthes bidentata Blume Seedlings under UV-B Radiation Stress L. Tanga, Y. Liua, S. Hanb, C. Maa, M. Zhanga, and J. Lia, c, * aCollege
of Life Sciences, Henan Normal University, Xinxiang, 453007 China School of Biological Sciences and Technology, Liupanshui Normal University, Liupanshui, 553004, China c Engineering Laboratory of Biotechnology for Green Medicinal Plant of Henan Province, Xinxiang, 453007 China *e-mail: [email protected] b
Received May 24, 2019; revised December 16, 2019; accepted February 17, 2020
Abstract—In this study, effects of soy isoflavones (SIF) on the growth and development, physiology, content of medicinal ingredients and expression levels of enzyme-encoding genes in Achyranthes bidentata Blume under UV-B radiation were systematically investigated. The results showed that UV-B radiation adversely affected the growth and development of A. bidentata seedlings, causing ROS accumulation and the anti-oxidation system to be damaged. However, 1 mg/L exogenous SIF treatment conferred enhanced tolerance under UV-B radiation stress in A. bidentata seedlings by decreasing the concentration of MDA, H2O2, and modulating SOD and POD activities. Furthermore, 1 mg/L SIF reduced the content of oleanolic acid and ecdysterone in leaves and increased the content of oleanolic acid in roots, but had no significant effect on the content of ecdysterone in roots under UV-B radiation stress. SIF had a different degree of influence on the expression levels of antioxidant enzyme genes as well as medicinal ingredients enzyme-encoding genes in A. bidentata. The results showed that 1 mg/L exogenous SIF treatment had a positive effect on the growth, development and physiology in A. bidentata seedlings under UV-B radiation stress. To our knowledge, for the first time we are reporting that the use of 1 mg/L exogenous SIF enhances A. bidentata seedlings tolerance to UV-B radiation stress. Keywords: Achyranthes bidentata, soy isoflavones, UV-B radiation, enzyme activity, gene expression DOI: 10.1134/S1021443720050179
INTRODUCTION Interaction between the earth’s biology and environment is currently the focus of widespread concern. Frequent human activities, to some extent, destroyed the ozone layer, resulting in an increase of UV-B radiation reaching the Earth’s surface [1]. Enhanced UV-B radiation intensity severely affects plants growth and development, physiological morphology, photosynthesis, secondary metabolism of the material, antioxidant system and inhibits key physiological processes so that to reduce plant biomass and yield [2–4]. Recent evidence suggests that DNA damage owing to increased UV-B radiation is a major cause of plants growth inhibition [3, 5]. The plants adapt themselves against a cerAbbreviations: CAS—cycloartenol synthase; CAT—catalase; FPS—farnesyl pyrophosphate synthase; MDA—malondialdehyde; OA—oleanolic acid; PBS—sodium phosphate buffer; POD—peroxidase; qRT-PCR—quantitative real-time PCR; SE—squalene oxi
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