Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China
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RESEARCH ARTICLE
Open Access
Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China Wen-qiao He1†, Yi-quan Xiong1,2†, Jing Ge1,3, Yan-xia Chen1, Xue-jiao Chen1, Xue-shan Zhong1, Ze-jin Ou1, Yu-han Gao1, Ming-ji Cheng1, Yun Mo1, Yu-qi Wen1, Min Qiu1, Shu-ting Huo1, Shao-wei Chen1, Xue-yan Zheng1, Huan He1, Yong-zhi Li1, Fang-fei You1, Min-yi Zhang1 and Qing Chen1*
Abstract Background: Rattus norvegicus and Suncus murinus are important reservoirs of zoonotic bacterial diseases. An understanding of the composition of gut and oropharynx bacteria in these animals is important for monitoring and preventing such diseases. We therefore examined gut and oropharynx bacterial composition in these animals in China. Results: Proteobacteria, Firmicutes and Bacteroidetes were the most abundant phyla in faecal and throat swab samples of both animals. However, the composition of the bacterial community differed significantly between sample types and animal species. Firmicutes exhibited the highest relative abundance in throat swab samples of R. norvegicus, followed by Proteobacteria and Bacteroidetes. In throat swab specimens of S. murinus, Proteobacteria was the predominant phylum, followed by Firmicutes and Bacteroidetes. Firmicutes showed the highest relative abundance in faecal specimens of R. norvegicus, followed by Bacteroidetes and Proteobacteria. Firmicutes and Proteobacteria had almost equal abundance in faecal specimens of S. murinus, with Bacteroidetes accounting for only 3.07%. The family Streptococcaceae was most common in throat swab samples of R. norvegicus, while Prevotellaceae was most common in its faecal samples. Pseudomonadaceae was the predominant family in throat swab samples of S. murinus, while Enterobacteriaceae was most common in faecal samples. We annotated 33.28% sequences from faecal samples of S. murinus as potential human pathogenic bacteria, approximately 3.06-fold those in R. norvegicus. Potential pathogenic bacteria annotated in throat swab samples of S. murinus were 1.35-fold those in R. norvegicus. Conclusions: Bacterial composition of throat swabs and faecal samples from R. norvegicus differed from those of S. murinus. Both species carried various pathogenic bacteria, therefore both should be closely monitored in the future, especially for S. murinus. Keywords: Rattus norvegicus, Suncus murinus, Bacterial composition, Next-generation sequencing
* Correspondence: [email protected] † Wen-qiao He and Yi-quan Xiong contributed equally to this work. 1 Department of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, 1838 Guangzhou North Road, Guangzhou 510515, China Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you
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