Faecal Excretion Dynamic during Subacute Oral Exposure to Different Pb Species in Rattus norvegicus
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Faecal Excretion Dynamic during Subacute Oral Exposure to Different Pb Species in Rattus norvegicus Zuzana Čadková & Jiřina Száková & Daniela Miholová & Petr Válek & Zuzana Pacáková & Jaroslav Vadlejch & Iva Langrová & Ivana Jankovská Received: 15 November 2012 / Accepted: 10 January 2013 / Published online: 14 February 2013 # Springer Science+Business Media New York 2013
Abstract Faecal excretion is a basic means of detoxification upon ingestion of Pb-contaminated feed. In order to determine a time course of Pb elimination after oral exposure to two different forms of this heavy metal (lead acetate vs. phytobound Pb), a feeding study was carried out in experimental rats using the Pb phyto-hyperaccumulator Pistia stratiotes as a model diet. The effect of starvation on Pb excretion was further studied in rats that were fed plant material. Twelve Pb doses (7 μg Pb/1 g BW) were administered orally over a 5week period. Faeces samples were collected 24 and 72 h postexposure. Inductively coupled plasma optical emission spectrometry and electrothermal absorption spectrometry methods were used for determination of heavy metal concentrations. Up to 53 % of ingested Pb was rapidly eliminated from the exposed rats via faeces within 24 h after exposure. Faecal excretion in exposed rats differed significantly when
compared to that of the control group. Fasting before exposure reduced Pb excretion by up to 50 %. Faecal excretions of both examined Pb forms exhibited almost identical patterns. Considerable differences were revealed concerning total excretion levels; lead acetate was excreted in amount greater extent than those of phytobound Pb. Results of our study suggest that Pb forms occurring in the P. stratiotes tissues are absorbed through the gastrointestinal tract to a greater extent than Pb from lead acetate. Therefore, higher portions of ingested Pb can be available for potential accumulation in tissues of exposed subjects.
Z. Čadková : P. Válek : J. Vadlejch : I. Langrová : I. Jankovská (*) Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, Prague 6 165-21, Czech Republic e-mail: [email protected]
Introduction
J. Száková Department of Agroenvironmental Chemistry and Plant Nutrition, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, Prague 6 165-21, Czech Republic D. Miholová Department of Chemistry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, Prague 6 165-21, Czech Republic Z. Pacáková Department of Stastistics, Faculty of Economics and Management, Czech University of Life Sciences Prague, Kamýcká 957, Prague 6 165-21, Czech Republic
Keywords Feeding study . Pb metabolism . Phytohyperaccumulator . Lead acetate . Excrement Pb level . Pistia stratiotes
Lead is the most abundant, persistent and widely distributed risk element in the environment, as confirmed by a United Nations Environment Programme [1] rep
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