Correlation between serum miR-154-5p and urinary albumin excretion rates in patients with type 2 diabetes mellitus: a cr
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RESEARCH ARTICLE
Correlation between serum miR-154-5p and urinary albumin excretion rates in patients with type 2 diabetes mellitus: a cross-sectional cohort study Huiwen Ren1,2, Can Wu3, Ying Shao4, Shuang Liu5, Yang Zhou1, Qiuyue Wang (
✉)1
1
Department of Endocrinology, the First Hospital of China Medical University, Shenyang 110001, China; 2Advanced Institute for Medical Sciences, Dalian Medical University, Dalian 116044, China; 3Department of Gastroenterology and Endoscopy, the First Hospital of China Medical University, Shenyang 110001, China; 4Department of Endocrinology, the Second Hospital of China Medical University, Shenyang 110001, China; 5Severe Infection Intensive Care Unit, Affiliated Central Hospital of Shenyang Medical College, Shenyang 110001, China
© Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract This study aimed to investigate the correlation between serum miR-154-5p and urinary albumin to creatinine ratio (UACR) in patients with type 2 diabetes mellitus (T2DM) and the association with biomarkers of inflammation and fibrosis in diabetic kidney disease (DKD). A total of 390 patients with T2DM were divided into three groups: normal albuminuria (UACR < 30 mg/g, n = 136, NA), microalbuminuria (UACR at 30–300 mg/g, n = 132, MA), and clinical albuminuria (UACR > 300 mg/g, n = 122, CA). Circulating miR-154-5p, inflammatory (C-reactive protein (CRP); erythrocyte sedimentation rate (ESR); and tumor necrosis factor-α (TNF-α) and fibrotic markers (vascular endothelial growth factor (VEGF); transforming growth factor-β1 (TGF-β1); and fibronectin (FN)), and other biochemical indicators were assessed via real-time PCR, enzyme-linked immunosorbent assay, and chemiluminescence assay in patients with T2DM and 138 control subjects (NC). UACR, miR-154-5p, glycated hemoglobin (HbA1c), serum creatinine (sCr), blood urea nitrogen (BUN), ESR, CRP, VEGF, TNF-α, TGF-β1, and FN were significantly higher and the estimated glomerular filtration rate (eGFR) was significantly lower in NA, MA, and CA groups than in NC subjects (P < 0.05). Elevated levels of UACR and miR-154-5p were directly correlated with HbA1c, sCr, BUN, ESR, CRP, VEGF, TNF-α, TGF-β1, and FN and negatively correlated with eGFR (P < 0.05). miR-154-5p, HbA1c, sCr, BUN, eGFR, ESR, CRP, VEGF, TNF-α, TGF-β1, and FN were important factors affecting UACR. These findings indicated that elevated serum miR-154-5p is significantly correlated with high UACR in patients with T2DM and may offer a novel reference for the early diagnosis of DKD. Keywords
type 2 diabetes mellitus; diabetic kidney disease; miR-154-5p; urinary albumin to creatinine ratio
Introduction Diabetic kidney disease (DKD), one of the complications of type 2 diabetes mellitus (T2DM), results from metabolic disorders. Its pathogenesis includes defects in the inflammatory response, immune system activation, and microvascular changes [1], which may eventually progress to end-stage renal disease (ESRD) [2–4]. However, the mechanisms underlying the occurrence and
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