Cottonseed meal fermented by Candida tropical reduces the fat deposition in white-feather broilers through cecum bacteri
- PDF / 801,101 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 68 Downloads / 149 Views
BIOTECHNOLOGICAL PRODUCTS AND PROCESS ENGINEERING
Cottonseed meal fermented by Candida tropical reduces the fat deposition in white-feather broilers through cecum bacteria-host metabolic cross-talk Junli Niu 1 & Jun Zhang 2 & Lianqing Wei 1 & Xi Ma 2 & Wenju Zhang 1 & Cunxi Nie 1,2 Received: 28 September 2019 / Revised: 29 February 2020 / Accepted: 11 March 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract In the present study, effects of cottonseed meal fermented by Candida tropicalis (FCSM) on fat deposition, cecum microbiota, and metabolites and their interactions were studied in broilers. A total of 180 1-day-old broilers were randomly assigned into two groups with six replicates of 15 birds in each. The birds were offered two diets consisted one control, i.e., supplemented with 0% FCSM (CON) and an experimental, with 6% FCSM (FCSM). Illumina MiSeq sequencing and liquid chromatography-mass spectrometry were used to investigate the profile changes of the cecum microbes and metabolites and the interactions among fat deposition, microbes, and metabolites. Results showed that at the age of 21 days, both the abdominal fat and subcutaneous fat thickness of the experimental birds decreased significantly (P < 0.05) in response to the dietary FCSM supplementation. The predominant microbial flora in cecum consisted Bacteroidetes (53.55%), Firmicutes (33.75%), and Proteobacteria (8.61%). FCSM diet increased the relative abundance of Bacteroides but decreased obese microbial including Faecalibacterium, Lachnospiraceae, Ruminococcaceae, and Anaerofilum. Cecum metabolomics analysis revealed that lipids, organic acids, vitamins, and peptides were significantly altered by adding FCSM in diet. Correlation analysis showed that abdominal fat and subcutaneous fat thickness related negatively with Bacteroides while the same related positively with Faecalibacterium, Lachnospiraceae, and Ruminococcaceae. Moreover, abdominal fat and subcutaneous fat thickness were related negatively with nicotinic acid, sebacic acid, thymidine, and succinic acid. These findings indicated that FCSM reduced the fat deposition by regulating cecum microbiota and metabolites in broilers. The results are contributory to the development of probiotics and the improvement in the production of broilers. Keywords Fungi . Fermented cottonseed meal . Lipid metabolism . Cecum microbiota . Cecum metabolites . Broiler
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00253-020-10538-7) contains supplementary material, which is available to authorized users. * Wenju Zhang [email protected] * Cunxi Nie [email protected] 1
College of Animal Science & Technology, Shihezi University, North Street 4, Xinjiang 832000, China
2
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, People’s Republic of China
In recent years, the obesity is a worldwide ongoing epidemic. Obese individuals usually suffer from
Data Loading...
