Coumarin-Based Reversible Fluorescent Probe for Selective Detection of Cu 2+ in Living Cells
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ORIGINAL ARTICLE
Coumarin-Based Reversible Fluorescent Probe for Selective Detection of Cu2+ in Living Cells Nadeem Ahmed 1 & Wajeeha Zareen 1 & Di Zhang 2 & Xiaopeng Yang 1 & Yong Ye 1 Received: 21 April 2020 / Accepted: 9 July 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Copper ion plays an important role in many biological processes in human body. H2S is considered as the third gasses transmitter after carbon monoxide and nitric oxide. Here a novel ICT-based fluorescent ON-OFF-ON probe for Cu2+ and H2S detection was developed. Selectivity and sensitivity of probe was confirmed in aqueous Tris-HCl buffer (10 mM, pH 7.4, containing 90% acetonitrile). Probe DF-CU shows high selectivity over other analytes. The degree of fluorescence quenching is linearly associated with the concentration of Cu2+ (R2 = 0.9919). The limit of detection (LOD, calculated according to the 3σ/slope) for Cu2+ was 6.4 μM. Probe can work in almost all pH. The probe shows a very fast response to Cu2+ (within 10 s). Its response to copper ion could be reversed by H2S. The complex of probe with Cu2+ could be used for H2S detection. Furthermore, this ON-OFF-ON fluorescent probe successfully applied in the living cells for the detection of Cu2+ and H2S. Keywords Fluorescent probe . Cu2+ . H2S . MCF-7 cells . Fluorescence imaging
Introduction Copper (Cu2+) is the third most abundant element found in the human body, along with zinc and iron. Cu2+ is present low in concentration in different tissue of the body, and the highest quantity of Cu2+ was found in the liver [1]. Cu2+ plays an efficient role in the transportation of cofactor of many Highlights 1. A new selective coumarin-based probe for Cu2+ detection was developed. 2. The probe showed a reverse response upon addition of H2S. 3. The probes were successfully applied to image Cu2+ and H2S in MCF7 Cells. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10895-020-02585-0) contains supplementary material, which is available to authorized users. * Di Zhang [email protected] * Yong Ye [email protected] 1
Green Catalysis Center, and College of Chemistry, Zhengzhou University, Zhengzhou 450001, China
2
Institute of Agricultural Quality Standards and Testing Technology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
proteins. In general, the concentration of copper ions in the blood are in the range of 100–150 mg/dL (15.7–23.6 mM) [2]. The limit of copper ion in drinking water was set to be 1.3 ppm (~20 mM) by the U.S. Environmental Protection Agency (EPA) [3]. Cu2+ has a significant role in cellular respiration, connective tissue establishment, bone growth, and response as a main catalytic cofactor for numerous metalloenzymes [4–11]. However, the exceeded amount of Cu2+ in the body may generate many reactive oxygen species (ROS) that result in damage to tissue and cells in the body. According to the reported, we known the disorder of copper ions in cells can cause some neurodegenerative d
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