Cytotoxic and Apoptotic Effects of Celecoxib and Topotecan on AGS and HEK 293 Cell Lines
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ORIGINAL RESEARCH
Cytotoxic and Apoptotic Effects of Celecoxib and Topotecan on AGS and HEK 293 Cell Lines Kimia Badalanloo 1
&
Tahereh Naji 1
&
Rahim Ahmadi 2
# Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Purpose This study is aimed to assess the anti-cancer effects of Celecoxib and topotecan against Human Gastric cancer cell line (AGS) in comparison to the control in an in-vitro study. Methods In this experimental study, Celecoxib and topotecan was prepared at concentrations of 500, 250, 125, 62.5, 31.2, 15.6 and 7.8 mg/ml. The effect of celecoxib and topotecan separately and in mixed form were investigated on AGS and normal HEK cells. To investigate the cell survival, MTT method was used to study the pathway of apoptosis using flowcytometry and Caspase kits based on colorimetric. Finally, one-way ANOVA and t-test were used to analyze the data. Results The results of this study indicated that Celecoxib was cytotoxic against AGS and HEK cell lines. The topotecan indicated a significant cytotoxicity against AGS cells and was not toxic against HEK cell line. Our results indicated that Celecoxib and topotecan have synergist effects against AGS and HEK cell lines and were more effective than separate celecoxib or topotecan. Conclusion The mixture of clecoxib and topotecan was more effective than celecoxib and topotecan in separate form. Our results indicated that use mixed forms of treatments can cause excellent therapeutic effects and can cause less side effects. Keywords Celecoxib . Topotecan . Gastric Cancer . Caspase . AGS . HEK
Background Gastric cancer is an vital health problem, is one of the most important type of cancer and is the second chief cause of death trough cancer in the world [1]. Around 950,000 new cases and about 700,000 death are yearly reported [2]. Dyspepsia, abdominal pain, weight loss and anorexia are the most common clinical signs for gastric cancer. Surgery is the gold standard curative therapeutic option, However, the use of residual adjuvant chemicals is also common to increase the survival of patients [3, 4]. Cisplatin, 5-fluorouracil, adriamycin, mitomycin; metrotexate; epirubicin, docetaxel, capecitabine; oxaliplatin are the most important drugs for Gastric cancer and most of them coincidences with adverse side * Tahereh Naji [email protected] 1
Department of Basic Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Islamic Azad University, Tehran, Iran
2
Department of Physiology, Faculty of Basic Sciences, Hamadan Branch, Islamic Azad University, Hamadan, Iran
effects [5]. Cyclooxygenase-2 (COX-2) plays an important role in tumor growth and proliferation that is normally absent in body tissues and the inhibitory effect of COX-2 on apoptosis induction has been reported in several studies [6, 7]. Furthermore, in vitro and in vivo studies have shown that inhibition of COX-2 enzyme inhibits the growth of gastric cancer and Given the initial role of COX-2 in gastric cancer, it seems reasonable to use its inhibitors in the treatm
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