Design and Evaluation of a Gas Chromatograph-Atmospheric Pressure Chemical Ionization Interface for an Exactive Orbitrap
- PDF / 2,693,698 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 20 Downloads / 178 Views
J. Am. Soc. Mass Spectrom. (2019) DOI: 10.1007/s13361-019-02311-6
RESEARCH ARTICLE
Design and Evaluation of a Gas Chromatograph-Atmospheric Pressure Chemical Ionization Interface for an Exactive Orbitrap Mass Spectrometer Joshua B. Powers,1,2 Shawn R. Campagna1,2 1
Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1600, USA Biological and Small Molecule Mass Spectrometry Core, University of Tennessee, Knoxville, TN 37996, USA
2
Abstract. Various separation and mass spectrometric (MS) techniques have furthered our ability to study complex mixtures, and the desire to meaOrbitrap sure every analyte in a system is of continual GC APCI interest. For many complex mixtures, such as the total molecular content of a cell, it is becoming OR apparent that no one single separation technique OR or analysis is likely to achieve this goal. Therefore, having a variety of tools to measure the complexity of these mixtures is prudent. Orbitrap MSs are broadly used in systems biology studies due to their unique performance characteristics. However, GC-Orbitraps have only recently become available, and instruments that can use gas chromatography (GC) cannot use liquid chromatography (LC) and vice versa. This limits small molecule analyses, such as those that would be employed for metabolomics, lipidomics, or toxicological studies. Thus, a simple, temporary interface was designed for a GC and Thermo Scientific™ Ion Max housing unit. This interface enables either GC or LC separation to be used on the same MS, an Exactive™ Plus Orbitrap, and utilizes an atmospheric pressure chemical ionization (APCI) source. The GC-APCI interface was tested against a commercially available atmospheric pressure photoionization (APPI) interface for three types of analytes that span the breadth of typical GC analyses: fatty acid methyl esters (FAMEs), polyaromatic hydrocarbons (PAHs), and saturated hydrocarbons. The GC-APCI-Orbitrap had similar or improved performance to the APPI and other reported methods in that it had a lower limit of quantitation, better signal to noise, and lower tendency to fragment analytes. Keywords: Gas chromatography, Atmospheric pressure chemical ionization (APCI), Atmospheric pressure chemical ionization (APPI), GC-MS, Fatty acid methyl esters (FAMEs), Polyaromatic hydrocarbons (PAHs), Saturated hydrocarbons, Interface, Orbitrap O
O
O
O
Received: 17 June 2019/Revised: 6 August 2019/Accepted: 6 August 2019
Introduction
A
s the ability to measure fully all analytes from complex mixtures has progressed, it has become apparent that two
Electronic supplementary material The online version of this article (https:// doi.org/10.1007/s13361-019-02311-6) contains supplementary material, which is available to authorized users. Correspondence to: Shawn Campagna; e-mail: [email protected]
main factors still hinder such efforts: The chemical diversity of these mixtures is high, making it difficult to probe the full complexity of the samples with a single analysis technique; and the identity of many analytes is
Data Loading...
