Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina
- PDF / 275,874 Bytes
- 5 Pages / 547.087 x 737.008 pts Page_size
- 64 Downloads / 150 Views
Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina Franco Daniel Fernández & Fabiana Aida Guzmán & Viviana Curzel & Noemí Bejarano & Luis Rogelio Conci
Accepted: 3 October 2012 / Published online: 18 October 2012 # KNPV 2012
Abstract Peach (Prunus persica L.) plants with symptoms of yellowing, reddening, curling and leaf necrosis, premature defoliation and internode shortening were observed in production fields in Jujuy province (Argentina). A phytoplasma was detected by PCR using the universal primer pairs P1/P7 and R16F2n/ R16R2 in all the symptomatic samples analysed. The RFLP profile of PCR products, amplified with R16F2n/R16R2 primers, shows that this phytoplasma, named Argentinean Peach Yellows (ArPY), belongs to subgroup 16Sr III-B. The phylogenetic analysis of the 1244 bp 16S rDNA cloned sequence, grouped the ArPY phytoplasma into the X-disease group with a Electronic supplementary material The online version of this article (doi:10.1007/s10658-012-0109-z) contains supplementary material, which is available to authorized users. F. D. Fernández : F. A. Guzmán : L. R. Conci (*) Instituto de Patología Vegetal, IPAVE-CIAP-INTA, Camino 60 cuadras Km 5 ½, X5020ICA Córdoba, Argentina e-mail: [email protected] V. Curzel : N. Bejarano Universidad Nacional de Jujuy, Facultad de Ciencias Agrarias-Laboratorio de Diagnóstico de enfermedades de las plantas, Jujuy, Argentina L. R. Conci Universidad Católica de Córdoba, Facultad de Ciencias Agropecuarias, Córdoba, Argentina
closer relationship with CFSD, PssWB and ChTDIII phytoplasmas. This is the first report of a phytoplasma infecting peach trees in Argentina. Keywords Argentina . Peach . Yellowing . PCR-RFLP . 16S rDNA
Argentina is one of the world’s ten largest peach producers. Most of the production comes from Rio Negro, Mendoza and Buenos Aires provinces, and new production areas have been recently incorporated. Among them is Jujuy province, placed in the NOA region (North West Argentina), with nearly 600 ha covered by peach orchards. Jujuy’s fruit production has significantly increased during the recent years focused mainly in early and extra-early maturing cultivars which are the first to reach the domestic market (Informe Frutihortícola 2006). During 2009–2011 peach trees with symptoms of leaf yellowing, reddening, curling necrosis, little leaf, premature defoliation, internode shortening, die back and progressive decline (Fig. 1) were observed in orchards located in temperate valleys of Jujuy. Leaf symptoms appeared in spring with chlorotic or necrotic leaf lesions, resulting in shot hole-like symptoms. Infected scaffold branches produced few smaller low quality and generally lack of embryo fruits with retarded development. Symptoms begin in young shoots of a branch and progressively spread to the entire
628
Eur J Plant Pathol (2013) 135:627–631
Fig. 1 Symptoms caused by Argentinean Peach Yellows phytoplasma in peach trees, a: leaf yellowing, reddening, curling and necrosis, b: internode shorten
Data Loading...
