Detection of multiple viruses in oropharyngeal samples from Brazilian free-tailed bats ( Tadarida brasiliensis ) using v
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BRIEF REPORT
Detection of multiple viruses in oropharyngeal samples from Brazilian free‑tailed bats (Tadarida brasiliensis) using viral metagenomics Samuel Paulo Cibulski1 · Francisco Esmaile de Sales Lima2 · Thais Fumaco Teixeira3 · Ana Paula Muterle Varela3 · Camila Mengue Scheffer3 · Fabiana Quoos Mayer4 · André Alberto Witt5 · Paulo Michel Roehe3 Received: 15 June 2020 / Accepted: 26 August 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract In this study, we analyzed the viral population in oropharyngeal samples from T. brasiliensis using a viral metagenomic approach. Genomes corresponding to members of the families Circoviridae, Genomoviridae, Herpesviridae, Paramyxoviridae, Coronaviridae, and Astroviridae were detected. This study provides the first preliminary understanding of the oropharyngeal virome of T. brasiliensis, which may guide the discovery and isolation of novel viruses in the future and highlights the need for continuing investigations in this regard. Bats (order Chiroptera) are one of the most diverse and widely distributed groups of mammals, representing ~ 20% of all known mammalian species [1]. Accompanying this diversity, it would not be unexpected to find an equally diverse microbiome within bat species. Bats are recognized as sources of viruses that can potentially cause disease in humans and animals; a myriad of viruses has been identified in bats of different species worldwide. Therefore, such species may be natural reservoirs for a large variety of potentially zoonotic viruses, such as lyssaviruses, paramyxoviruses, and filoviruses as well as the recently emerged severe
Handling Editor: Akbar Dastjerdi. Samuel Paulo Cibulski and Francisco Esmaile de Sales Lima have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04825-x) contains supplementary material, which is available to authorized users.
acute respiratory syndrome coronavirus 2 (SARS-CoV-2) [2]. Since nearly 80% of viral diseases that infect humans are zoonotic, field surveys in search for viruses must be conducted by monitoring such populations in their usual – or adopted – habitats [3]. Therefore, knowledge about the ecology of the potential reservoir species and main routes of interspecies transmission is central to any preventive campaign. T. brasiliensis is one of the most widely distributed mammalian species in the Western Hemisphere and is highly adapted to urban environments. In Brazil, this insectivorous species is known to harbor some important pathogens, such as rabies virus and coronaviruses. However, the actual amplitude of the virome of this bat species remains undetermined. In the present study, oropharyngeal swabs samples from 155 healthy Brazilian free-tailed bats were collected. The choice of this sample collection was based on the fact that this procedure is widely accepted as sampling method for
* Samuel Paulo Cibulski [email protected] 1
Centro de Biotecnologia‑CBiotec, Laboratóri
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