Multiple DNA viruses identified in multimammate mouse ( Mastomys natalensis ) populations from across regions of sub-Sah
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ORIGINAL ARTICLE
Multiple DNA viruses identified in multimammate mouse (Mastomys natalensis) populations from across regions of sub‑Saharan Africa Sébastien Calvignac‑Spencer1 · Léonce Kouadio2,3 · Emmanuel Couacy‑Hymann2 · Nafomon Sogoba4 · Kyle Rosenke5 · Andrew J. Davison6 · Fabian Leendertz3 · Michael A. Jarvis7,8 · Heinz Feldmann5 · Bernhard Ehlers9 Received: 25 February 2020 / Accepted: 17 June 2020 / Published online: 4 August 2020 © The Author(s) 2020
Abstract The multimammate mouse (Mastomys natalensis; M. natalensis) serves as the main reservoir for the zoonotic arenavirus Lassa virus (LASV), and this has led to considerable investigation into the distribution of LASV and other related arenaviruses in this host species. In contrast to the situation with arenaviruses, the presence of other viruses in M. natalensis remains largely unexplored. In this study, herpesviruses and polyomaviruses were identified and partially characterized by PCR methods, sequencing, and phylogenetic analysis. In tissues sampled from M. natalensis populations in Côte d’Ivoire and Mali, six new DNA viruses (four betaherpesviruses, one gammaherpesvirus and one polyomavirus) were identified. Phylogenetic analysis based on glycoprotein B amino acid sequences showed that the herpesviruses clustered with cytomegaloviruses and rhadinoviruses of multiple rodent species. The complete circular genome of the newly identified polyomavirus was amplified by PCR. Amino acid sequence analysis of the large T antigen or VP1 showed that this virus clustered with a known polyomavirus from a house mouse (species Mus musculus polyomavirus 1). These two polyomaviruses form a clade with other rodent polyomaviruses, and the newly identified virus represents the third known polyomavirus of M. natalensis. This study represents the first identification of herpesviruses and the discovery of a novel polyomavirus in M. natalensis. In contrast to arenaviruses, we anticipate that these newly identified viruses represent a low zoonotic risk due to the normally highly restricted specificity of members of these two DNA virus families to their individual mammalian host species. Abbreviations BMCMC Bayesian Markov chain Monte Carlo CI Côte d’Ivoire DPOL DNA polymerase Handling editor: Akbar Dastjerdi. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04738-9) contains supplementary material, which is available to authorized users. * Bernhard Ehlers [email protected] 1
LASV Lassa virus gB Glycoprotein B LD-PCR Long-distance PCR LTAg Large T antigen MCPyV Merkel cell polyomavirus ML Maximum likelihood M. natalensis Mastomys natalensis MnatCMV Mastomys natalensis cytomegalovirus MnatPyV Mastomys natalensis polyomavirus 5
Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA
P3 “Viral Evolution”, Robert Koch-Institute, Berlin, Germany
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LANADA/Central Laboratory for Animal Disea
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