Determination of Cyantraniliprole and its Major Metabolite Residues in Pakchoi and Soil Using Ultra-Performance Liquid C
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Determination of Cyantraniliprole and its Major Metabolite Residues in Pakchoi and Soil Using Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry Jianpeng Sun • Nan Feng • Congfeng Tang Dongmei Qin
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Received: 3 June 2012 / Accepted: 11 July 2012 / Published online: 30 August 2012 Ó Springer Science+Business Media, LLC 2012
Abstract A rapid, simple and reliable analytical method was developed for the determination of cyantraniliprole and its major metabolite J9Z38 in pakchoi and soil by using ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). The sample preparation approach is known as QuEChERS, which stands for quick, easy, cheap, effective, rugged and safe. Samples were extracted with acetonitrile, and cleaned up with dispersive primary and secondary amine sorbent before analysis by UPLC–MS/MS. The limit of quantitation for cyantraniliprole and J9Z38 was 0.01 mg/kg in both pakchoi and soil. Average recoveries of cyantraniliprole and J9Z38 at three fortified levels (0.01, 0.05, 0.1 mg/kg) ranged from 77.8 % to 102.5 % with relative standard deviation of 1.6 %– 8.9 %. This method has been applied to the analysis of cyantraniliprole and J9Z38 residues in real pakchoi and soil samples selected from field. The results of the residue dynamic experiment showed that the half-life of cyantraniliprole ranged from 2.9 to 6.4 days in pakchoi and 8.7 to 18.2 days in soil, respectively. The final residual levels of cyantraniliprole in pakchoi and soil from Guangdong and Shanghai were below 0.20 and 0.10 mg/kg, respectively; similarly, the final residual levels of J9Z38 in pakchoi and J. Sun D. Qin (&) Institute for the Control of Agrochemicals, Ministry of Agriculture, Beijing 10026, People’s Republic of China e-mail: [email protected] N. Feng Beijing Municipal Center for Food Safety Monitoring, Beijing 100041, People’s Republic of China C. Tang State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People’s Republic of China
soil from Guangdong and Shanghai were \0.07 and 0.01 mg/kg. These results will be helpful in setting maximum residue limit guidance for cyantraniliprole in pakchoi in China. Keywords Cyantraniliprole J9Z38 UPLC–MS/MS Residue Pakchoi
Cyantraniliprole(3-bromo-1-(3-chloro-2-pyridinyl)-N-[4cyano-2-methyl-6-[(methyl-amino)carbonyl]phenyl]-1Hpyrazole-5-carboxamide, Fig. 1a) is an anthranilic diamide insecticide developed by DuPont Crop Protection that activate insect ryanodine receptors, causing calcium ions to pass rapidly out of muscle cells, resulting in feeding cessation followed by muscle paralysis, lethargy and regurgitation by affected insects (Jacobson and Kennedy 2011; Timo et al. 2011). Cyantraniliprole shows exceptional insecticidal activity on a broad range of Lepidopera, Coleoptera, Diptera, and Isoptera insects, and it is reported to be active against a broader spectrum of insects than chlorantraniliprole (Chai et al. 2010). To the best of our knowledge,
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