Determination of Eleutheroside B and Eleutheroside E in Extracts from Eleutherococcus senticosus by Liquid Chromatograph
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Determination of Eleutheroside B and Eleutheroside E in Extracts from Eleutherococcus senticosus by Liquid Chromatography/Mass Spectrometry D. I. Baygildievaa, *, A. V. Brauna, A. N. Stavrianidia, and I. A. Rodina aLomonosov
Moscow State University, Chemistry Department, Moscow, 119991 Russia *e-mail: [email protected] Received August 1, 2019; revised August 29, 2019
Abstract—Eleutherococcus senticosus is a plant with medicinal properties due to the presence of glycosides, called eleutherosides А, В and В1, C, D, E, and F, in its roots and rhizomes. In this paper, a procedure for the simultaneous determination of Eleutheroside B and Eleutheroside E in dry samples of Eleutherococcus senticosus roots from different manufactures by high performance liquid chromatography/tandem mass spectrometry was proposed. The quantification of eleutheroside B and eleutheroside E was performed using electrospray ionization in the negative ion mode with detection by multiple reaction monitoring (MRM), which ensured a better signal-to-noise ratio. To isolate eleutherosides from plant materials, simple and rapid methods of sample preparation were used, including extraction in an ultrasonic bath with various solvents. The calibration curve was linear in the concentration range from 40 to 4000 ng/mL. Keywords: eleutherosides B and E, Eleutherococcus senticosus, plant extracts, high performance liquid chromatography/mass spectrometry, electrospray ionization DOI: 10.1134/S1061934820140051
INTRODUCTION Eleutherococcus senticosus (Rupr. Et Maxim.) (Eleutherococcus prickly, or freeberry prickly, or wild pepper, or devil’s bush) is a shrub plant widespread in the Northeast Asia and Siberia. Eleutherococcus contains a wide range of pharmacologically active compounds, such as diterpenoids [1], triterpenoids [2], saponins [3], phenylpropanoids [4], flavones, and coumarins [5]. In addition, it has been widely used in traditional folk medicine to treat rheumatism, diabetes, and hepatitis [6]. Eleutherococcus senticosus is characterized by eleutherosides, belonging to the class of triterpene glycosides [7]. Eleutherosides are a group of diverse compounds isolated from the roots of the Eleutherococcus senticosus plant. Eleutheroside B (beta-D-glucopyranoside, 4-[(1E)-3-hydroxy-1-propenyl]-2,6-dimethoxyphenyl), a compound found in the rhizomes of the Acanthopanax senticosus plant, is widely used in traditional Chinese medicine. In the course of medical research it was found that eleutheroside B can reduce fatigue and possesses anticoagulant properties, which determines the adaptogenic properties of extracts from eleutherococcus [7]. The structural formula of eleutheroside B is shown in Fig. 1a. Eleutheroside E ((2R,3S,4R,5R,6S)-2-[4-[4-[3,5Dimethoxy-4-[(2S,3R,4S,5S,6R)-3.4.5-trihydroxy-6-
(hydroxymethyl) oxan-2-yl]oxyphenyl]-1,3,3a,4,6,6ahexahydrofuro[4,3-c]furan-1-yl]-2,6-dimethoxyphenoxy]-6-(hydroxymethyl)oxane-3,4,5-triol) is the main biologically active component of Eleutherococcus senticosus, widely used in traditional Chinese medici
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