Determination of Menbutone in Bovine Milk and Meat Using Micellar Liquid Chromatography: Application to Injectable Dosag
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Determination of Menbutone in Bovine Milk and Meat Using Micellar Liquid Chromatography: Application to Injectable Dosage Forms F. Belal 1 & Sawsan A. Abd El-Razeq 2 & Manal M. Fouad 2 & S. Zayed 3 & Fatma A. Fouad 2
Received: 23 February 2015 / Accepted: 10 June 2015 # Springer Science+Business Media New York 2015
Abstract A simple, sensitive, and rapid method was developed for the routine identification and quantification of menbutone in different matrices by micellar liquid chromatography. Separation was performed in less than 4 min using a C18 column with UV detection at 234 nm. A micellar solution composed of 0.12 M sodium dodecyl sulfate, 8 % n-butanol, and 0.3 % triethylamine in 0.02 M phosphoric acid at pH 6.0 was used as the mobile phase. The method was fully validated in accordance with International Conference on Harmonization (ICH) guidelines. The limits of detection and quantitation were 0.95 and 2.86 ng mL−1, respectively. The method showed good repeatability, linearity, and sensitivity according to the evaluation of the validation parameters. The micellar method was successfully applied for the analysis of menbutone in its commercial injections with a mean % recovery value of 99.73± 1.634 % and in spiked bovine milk and meat samples with a mean % recovery values in the range of 98.00–100.60 %. High extraction efficiency was obtained without matrix interference in the extraction process and in the subsequent chromatographic determination. No organic solvent was used during the pretreatment step. Hence, this method can be considered as an interesting example for green chemistry.
* S. Zayed [email protected] 1
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, University of Mansoura, Mansoura 35516, Egypt
2
Analytical Chemistry Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt
3
Unit of Drug Analysis, Faculty of Pharmacy, University of Mansoura, Mansoura 35516, Egypt
Keywords Micellar liquid chromatography . Menbutone . Dosage forms . Bovine milk . Meat
Introduction Menbutone (4-methoxy-γ-oxo-1-naphthalene butanoic acid) (Fig. 1) (O’Neil 2006) is a specific stimulant of exocrine glands of the digestive tract: digestive tonic and choloreticin bovine, ovine, porcine, and equine species (Symonds 1982). It helps in any course affecting the digestive system as diarrhea, anorexia, and gastroenteritis (Ackerman 2007). After injection into the body, it increases biliary, pancreatic, and peptic secretion by two to five times compared with the normal levels of these secretions. In modern farming practice, drugs are used in a large scale and are applied in animal husbandry for different reasons. They are used to prevent diseases, cure animals, or as feed additive to promote growth. In the veterinary clinic, animals digestive diseases account for about 30 % of the total number of clinical diseases and loss of appetite, indigestion, constipation, and other gastrointestinal dysfunctions are almost concomitant diseases or secondary in most animals. Animal indigestio
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