Development and application of a colloidal carbon test strip for the detection of antibodies against Mycoplasma bovis
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(2020) 36:157
ORIGINAL PAPER
Development and application of a colloidal carbon test strip for the detection of antibodies against Mycoplasma bovis Feng Shi1 · Yang Zhao2 · Yixiao Sun1 · Chuangfu Chen2 Received: 9 December 2019 / Accepted: 14 September 2020 © Springer Nature B.V. 2020
Abstract Mycoplasma bovis (M. bovis) is an important bovine mycoplasma implicated in economically important clinical diseases, such as respiratory diseases, otitis media, and mastitis. The prevalence of M. bovis-associated mastitis in both cattle and buffaloes has been increasingly recognized as a global problem. High morbidity rates and consequential economic losses have been devastating to the affected cattle and buffalo farms, especially those in developing countries. Therefore, a rapid and accurate method is urgently needed to detect M. bovis. In this study, a rapid and simple lateral flow strip for detecting antibodies against M. bovis was established that used carbon nanoparticles (CNPs) as the labelled materials. The results from the test strip were highly consistent with those from ELISA. The test showed high specificity (100%) and no cross-reaction with other bovine pathogens. The detection sensitivity of the test was also relatively high (97.67%). All the results indicated that the colloidal carbon test strip could serve as a simple, rapid, sensitive, and specific diagnostic method for detecting antibodies against M. bovis at cattle farms. Keywords Colloidal carbon · ELISA · Lateral flow · Mycoplasma bovis · Visual detection
Introduction Mycoplasma bovis, a prokaryotic microorganism that lacks a cell wall, causes severe pneumonia, mastitis, arthritis, otitis media, and reproductive disorders in cattle. It also causes exudative pleuritic tuberculous in humans (Fox et al. 2005; Kay et al. 2015). M. bovis is mainly transmitted by droplets from the respiratory tract (Foster et al. 2009). M. bovis mainly spreads by droplets ejected from the respiratory tract, and secondly by contacts. It often causes acute and chronic diseases in cattle in a direct or indirect way (Ball and Nicholas 2010). The development of disease severely affects the cattle industry by increasing the cost of feeding and the Feng Shi and Yang Zhao have contributed equally to this work. * Feng Shi [email protected] * Chuangfu Chen ccf‑[email protected] 1
College of Life Science, Shihezi University, Shihezi, Xinjiang 832003, P. R. China
College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, P. R. China
2
costs associated with the diagnosis and treatment of disease, including permanent lung injury (Ayling et al. 2014). Traditionally, the identification and diagnosis of M. bovis has been performed via microbial culture. (Hazelton et al. 2018; Parker et al. 2018; Zhao et al. 2018). More recently, the use of polymerase chain reaction (PCR) to detect M. bovis species from various bovine samples has increased. PCR has a higher efficiency, specificity, and sensitivity for laboratory diagnosis when compared with conventional c
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