Development of a rapid and sensitive europium (III) chelate microparticle-based lateral flow test strip for the detectio
- PDF / 1,609,962 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 3 Downloads / 178 Views
ORIGINAL ARTICLE
Development of a rapid and sensitive europium (III) chelate microparticle‑based lateral flow test strip for the detection and epidemiological surveillance of porcine epidemic diarrhea virus Jianbo Liu1 · Hongyan Shi1 · Guangyi Cong1 · Jianfei Chen1 · Xin Zhang1 · Da Shi1 · Liyan Cao1 · Xiaobo Wang1 · Jialin Zhang1 · Zhaoyang Ji1 · Zhaoyang Jing1 · Li Feng1 Received: 10 October 2019 / Accepted: 28 January 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, is the predominant cause of severe enteropathogenic diarrhea in swine. A simple, rapid, specific, and sensitive method is critical for monitoring PEDV on pig farms. In this study, a simple and rapid lateral flow immunoassay detection system that integrates europium (Eu) (III) chelate microparticles was developed to identify PEDV in fecal swabs. This newly developed diagnostic sandwich immunoassay utilizes lateral flow test strips (LFTSs). The fluorescence peak heights of the test line (HT) and the control line (HC) were measured using a fluorescence strip reader, and the HT/HC ratio was used for quantitation. The limit of detection of PEDV with this LFTS was ??ten times the median tissue culture infectious dose ( TCID50) per mL??. Fecal swab samples were used to determine the cutoff value. Field samples, various PEDV strains and other viruses were used to determine the sensitivity and specificity of the Eu (III) chelate microparticle-based LFTSs, which were 97.8% and 100%, respectively, with a cutoff value of 0.05, as compared with reverse transcription polymerase chain reaction (RT-PCR). In samples from piglets experimentally infected with PEDV, the results were in high agreement with those obtained by RT-PCR. Epidemiological surveillance of PEDV using the LFTSs ??in areas threatened by African swine fever virus?? suggested that the PEDV positive rate on pig farms had significantly decreased, mainly due to the implementation of strict biosecurity measures. The results indicate that the Eu (III) chelate microparticle-based LFTS system is a rapid, sensitive, and reliable method for the identification of PEDV, indicating its suitability for epidemiological surveillance of PEDV infection.
Introduction Enteric disease associated with porcine epidemic diarrhea virus (PEDV) was first reported in pigs in England in 1971 [20]. Since then, PEDV has spread to other European and Asian countries [23], as well as to North America in 2013 [8]. In China, PEDV was first confirmed in 1984, and since 2010, it has caused enteric disease, which is characterized by high morbidity and mortality in pre-weaning piglets, resulting in serious economic losses to the swine industry Handling Editor: Diego G. Diel. * Li Feng [email protected] 1
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, 678 Haping Road, Xiangfang District, Harbin 150069, China
nationwide [5, 14]. After the initia
Data Loading...
