Development and Evaluation of a Disposable Solid-State Culture Packed-Bed Bioreactor for the Production of Conidia from
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ORIGINAL PAPER
Development and Evaluation of a Disposable Solid‑State Culture Packed‑Bed Bioreactor for the Production of Conidia from Trichoderma asperellum Grown Under Water Stress Ynoussa Maïga1,2 · Quentin Carboué1 · Rayhane Hamrouni1,3 · Marie‑Stéphane Tranier1 · Yassine Ben Menadi1,4 · Sevastianos Roussos1 Received: 7 April 2020 / Accepted: 13 August 2020 © Springer Nature B.V. 2020
Abstract A new prototype of a disposable solid-state culture bioreactor (DSSCB) belonging to the packed-bed category is developed for the production of fungal conidia. The bioreactor has the advantages of being autoclavable, lightweight and disposable. With a working volume of 39.52 L, it can be used for large-scale production of fungal conidia. The DSSCB was successfully tested under forced aeration for the production of conidia (2.1 × 1010/g dry substrate in 140 h) from Trichoderma asperellum, a biocontrol fungus. A mixture of sugarcane bagasse, wheat bran, olive pomace, potato flakes and vine shoots was used as substrate. The DSSCB allowed an overall production of 1.26 × 1014 conidia. Based on its configuration, the fermented substrate can be easily transported and used in either solid or liquid form, for biocontrol application. This is a significant advance in the field of biomass production in solid-state culture as problems associated with the decontamination of the bioreactor prior to its reuse can be avoided. The study demonstrated that the DSSCB is suitable for the production of conidia using T. asperellum. Graphic Abstract
Fungal conidia Agro-industrial residues
DSSCB filled with waste residues and fungal conidia
Fermented substrate in the DSSCB
Conidia of Trichoderma asperellum Keywords Conidia · Solid-state culture · Trichoderma asperellum · Water stress
* Ynoussa Maïga [email protected] Extended author information available on the last page of the article
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Statement of Novelty Many bioreactors have been developed for the production of bio-active products; however, they are limited by their small size or by difficulties linked to their operation and maintenance. This study reports the development of a disposable solid-state culture bioreactor (DSSCB) with a working volume of 39.52 L, allowing the easy transport of fermented products which can be used in either solid or liquid form, for application in remote farmlands. The DSSCB successfully and massively produced conidia of Trichoderma asperellum, an important biocontrol agent. This is the first report of conidia production in solid-state culture using a disposable bioreactor working at this scale (18 kg of wet medium).
Introduction Solid-state culture (SSC) is generally defined as the culture of microorganisms on a solid medium in the absence (or near absence) of free water, but with enough moisture to support the growth and metabolism of the microorganisms [1, 2]. When it uses agro-industrial residues as substrates for the production of bio-active products of commercial interest, this process can be classified as a waste ma
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