Optimization of Nutrient Requirements and Culture Conditions for the Production of Rhamnolipid from Pseudomonas aerugino
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ORIGINAL ARTICLE
Optimization of Nutrient Requirements and Culture Conditions for the Production of Rhamnolipid from Pseudomonas aeruginosa (MTCC 7815) using Mesua ferrea Seed Oil Salam Pradeep Singh • Pranjal Bharali Bolin Kumar Konwar
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Received: 5 December 2012 / Accepted: 28 March 2013 Ó Association of Microbiologists of India 2013
Abstract Environmental awareness has led to a serious consideration for biological surfactants and hence nonedible vegetable oils may serve as a substitute carbon source for bio-surfactant production (rhamnolipid) which might be an alternative to complex synthetic surfactants. There are reports of rhamnolipid production from plant based oil giving higher production than that of glucose because of their hydrophobicity and high carbon content. Therefore the contribution of non-edible oil such as Mesua ferrea seed oil could serve as a good carbon source for rhamnolipid production. Moreover the use of rhamnolipid production from non-edible plant based seed oil has not been reported elsewhere. The present work focus on the optimal production of rhamnolipid by considering both micro and macro nutrients and culture conditions using response surface methodology. The study observes that micronutrients play a significant role in rhamnolipid production from Pseudomonas aeruginosa (MTCC 7815). The investigation results with the statistically optimize parameters able to produce a higher rhamnolipid production and this methodology could be used to optimize the nutrients requirements and culture conditions. The present findings would assist in bioremediation of crude oil contaminated ecosystems. Keywords Rhamnolipid Non-edible oil Response surface methodology Carbon source Electronic supplementary material The online version of this article (doi:10.1007/s12088-013-0403-2) contains supplementary material, which is available to authorized users. S. P. Singh (&) P. Bharali B. K. Konwar Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur 784028, Assam, India e-mail: [email protected]
Introduction Environmental awareness has led to a serious circumstance realizing biological surfactants as an alternative to synthetic surfactants [1]. Biosurfactants have higher specificity, low toxicity, higher biodegradability, stability at extreme pH, salinity and temperature compared to synthetic surfactants. Their unique structures provide unique properties that lacks in commercial surfactants [2]. Moreover, it has application in petroleum industry which includes enhanced oil recovery (EOR), bioremediation and waste treatment to remove hazardous materials, pharmaceutical-cosmetics formulas, agriculture, food processing industries, detergents, laundry supplies and paint industries [3–6]. Recently, biosurfactants have received much attention in nanobiotechnology too [7, 8]. Despite their potential applications, they are not employed extensively in industry because of its high production cost associated with inefficient methods for product recovery and expensive subst
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