Development of a Flexible MIP-Based Biosensor Platform for the Thermal Detection of Neurotransmitters
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MRS Advances © 2017 Materials Research Society DOI: 10.1557/adv.2017.634
Development of a Flexible MIP-Based Biosensor Platform for the Thermal Detection of Neurotransmitters Kai Betlem1, Michael P. Down1, Christopher W. Foster1, Shamima Akthar1, K. Eersels2, B. van Grinsven2, T.J. Cleij2, C.E. Banks1, M. Peeters1* 1) Manchester Metropolitan University, Faculty of Science and Engineering, Div. of Chemistry & Environmental Science, John Dalton Building, Chester Street, M15GD, Manchester, United Kingdom. 2) Maastricht University, Maastricht Science Programme, P.O. Box 616, 6200 MD Maastricht, The Netherlands.
ABSTRACT
We have developed high affinity Molecularly Imprinted Polymers (MIPs) for neurotransmitters such as dopamine, noradrenaline and caffeine. These polymer particles are mixed within the bulk of screen-printed ink allowing masss-producible bulk modified MIP Screen-Printed Electrodes (MIP-SPEs) to be realised. We have explored different SPE supporting surfaces, such as polyester, tracing paper and household-printing paper. The performance of those MIP-SPEs is studied using the Heat-Transfer Method (HTM), a patented thermal method. With the combination of screen-printing techniques and thermal detection, it is possible to develop a portable sensor platform that is capable of low-cost and straightforward detection of biomolecules on-site. In the future, this unique sensor architecture holds great promise for the use in biomedical devices.
INTRODUCTION Molecularly Imprinted Polymers (MIPs) are synthetic antibody mimics; similar to antibodies, they have high affinity for a chosen template molecule but their advantages include low-cost, superior chemical and thermal stability, and straightforward production process [1,2]. These polymeric receptors are widely used in purification and separation processes but have limited applications in the field of biosensors [3]. Key challenges in that area include difficulty to incorporate MIPs into suitable sensor platforms and the lack of low-cost and straightforward sensing strategies. In this contribution, we will focus on using SPEs as sensor platforms since they are highly reproducible, can be used as disposable electrodes and their production process can be tailored with our in-house facilities. We were the first to report on novel and simple functionalization strategy for the direct incorporation of MIPs into SPEs [4]. These MIP-SPEs will be combined with the Heat-Transfer Method (HTM) as sensing strategy. HTM is a patented thermal analysis technique [5] that relies on evaluating the thermal resistance at the solid-liquid interface. This method has commercial potential
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but is not currently suitable for mass-production due to various reasons, including the use of home-made design of the thermocouple device and flow cells. We
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