Development of a green and efficient methodology for the heterocyclic aromatic amine determination in biomass samples ge
- PDF / 1,186,343 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 91 Downloads / 195 Views
RESEARCH ARTICLE
Development of a green and efficient methodology for the heterocyclic aromatic amine determination in biomass samples generated from cigarette combustion and tobacco Romina Canales 1 & Maria Guiñez 1 & Carolina Talio 1 & Mario Reta 2 & Soledad Cerutti 1 Received: 29 May 2020 / Accepted: 7 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A green methodology was developed for the analysis of ten heterocyclic aromatic amines (HAAs) in biomass samples from cigarette combustion such as mainstream smoke, paper ashes, as well as tobacco and paper wraps. The cellulose filter used for sample collection was also evaluated. This strategy was based on ultrasound-assisted extraction (UAE) associated with a solidphase extraction procedure employing multi-walled carbon nanotubes (MWCNTs-SPE) as a cleanup step followed by ultra-highperformance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Under optimal experimental conditions, the linearity of the method was in the range from 0.08 to 160 ng cig-1, with correlation coefficients (R2) higher than 0.991. The limits of detection resulted to be between 0.03 and 0.63 ng cig-1. Concentrations of the HAAs in the mainstream smoke were from 5.7 to 145.2 ng cig-1 and in paper ashes from 0.1 to 0.6 ng cig -1, while in tobacco were between 1.0 and 38.5 ng cig-1. Meanwhile, no HAA contribution was observed in the case of paper wraps and the filter used for sample collection. The knowledge of the presence and the concentration levels of the selected HAAs in each cigarette’s physical component after its combustion is essential to understand the formation processes and contribution during cigarette burning. Besides, this is the first report about the presence of some HAAs in the proposed samples. Finally, a comparative study was employed to classify the sustainability of several recent approaches for HAA extraction from cigarette combustion samples using Green Certificate as a metric tool. Keywords Heterocyclic aromatic amines . UAE-MWCNT-based SPE . Mainstream smoke . Paper ashes . Tobacco . Green Certificate
Introduction
Responsible editor: Ester Heath Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11356-020-10759-3) contains supplementary material, which is available to authorized users. * Soledad Cerutti [email protected] 1
Instituto de Química de San Luis, Facultad de Química, Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Luis, Bioquímica y Farmacia Bloque III, Avda. Ejército de los Andes 950, D5700BPB San Luis, Argentina
2
Laboratorio de Investigación y Desarrollo de Métodos Analíticos (LIDMA), División Química Analítica, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 47 y 115, (B1900AJL), La Plata, Argentina
Tobacco use is associated with the main cause of preventable death (WHO 2013). Cigarette smoke presents more than 8000 chemical hazardous compounds evidencing an acutely complex composition (Rodgman and Per
Data Loading...