Development of an Accelerated Solvent Extraction Approach for Quantitative Analysis of Chloramphenicol, Thiamphenicol, F
- PDF / 724,596 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 89 Downloads / 246 Views
Development of an Accelerated Solvent Extraction Approach for Quantitative Analysis of Chloramphenicol, Thiamphenicol, Florfenicol, and Florfenicol Amine in Poultry Eggs Bo Wang 1,2 & Xia Zhao 2,3 & Xing Xie 4 & Kaizhou Xie 2,3 & Genxi Zhang 2,3 & Tao Zhang 2,3 & Xuezhong Liu 1,2 Received: 22 November 2018 / Accepted: 22 April 2019 / Published online: 4 May 2019 # Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract This manuscript describes a simple, reliable, and sensitive approach that utilizes high-performance liquid chromatographyelectrospray ionization tandem triple quadrupole mass spectrometry (HPLC-ESI/MS/MS) for the selective determination of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in poultry eggs (hen eggs, duck eggs, and goose eggs). Samples were extracted with methanol-ammonium hydroxide-ultrapure water (97:2:1, v/v) via accelerated solvent extraction (ASE) at 80 °C and 1500 psi and defatted with hexane saturated with acetonitrile. The experimental method uses ESI (+) and ESI (−) combination mode, full-scan qualitative analysis, and multiple reaction monitoring (MRM) combined with an internal standard for quantitative analysis. This optimized method was validated according to the requirements defined by the European Union and the Food and Drug Administration. The poultry eggs were spiked with the target compounds at four levels, and the average recoveries were all higher than 88.3%, and the relative standard deviations did not exceed 3.9%. The limits of detection and limits of quantification were 0.04–0.5 μg/kg and 0.1–1.5 μg/kg, respectively. The decision limits were 0.37– 102 μg/kg, and the detection capabilities were 0.44–103 μg/kg. Finally, the new approach was successfully applied to the quantitative determination of these analytes in 120 commercial poultry eggs from local supermarkets. Keywords Poultry eggs . Chloramphenicol . Thiamphenicol . Florfenicol . Florfenicol amine . HPLC-ESI/MS/MS
Introduction Chloramphenicol (CAP), thiamphenicol (TAP), and florfenicol (FF) belong to the same class of chloramphenicol drugs (CAPs), which have similar antibacterial mechanisms and have effects on both Gram-positive and Gram-negative bacteria. Their effects on Gram-negative bacteria are stronger than those on Gram-positive bacteria, and these drugs are
* Kaizhou Xie [email protected] 1
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
2
Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China
3
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
4
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China
widely used in livestock production (Holt et al. 1998; Festing et al. 2001; Du et al. 2004; Graziani et al. 2008). However, chloramphenicol can cause aplastic anemia in humans and animals; thiamphe
Data Loading...
