Development of nuclear microsatellite markers to facilitate germplasm conservation and population genetics studies of fi
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SHORT COMMUNICATION
Development of nuclear microsatellite markers to facilitate germplasm conservation and population genetics studies of five groups of tropical perennial plants with edible fruits and shoots: rambutan (Nephelium lappaceum L.), sapodilla (Manilkara zapota (L.) P. Royen), lychee (Litchi chinensis Sonn.), mangosteen (Garcinia mangostana Linn. and Garcinia cochinchinensis (Lour.) Choisy) and bamboo (Bambusa vulgaris Schrad. ex J.C. Wendl and Guadua angustifolia Kunth) Rene´e S. Arias . Linda L. Ballard . Mary V. Duke . Sheron A. Simpson . Xiaofen F. Liu . Valerie A. Orner . Victor S. Sobolev . Brian E. Scheffler . Jaime Martinez-Castillo Received: 13 March 2020 / Accepted: 15 June 2020 Ó Springer Nature B.V. 2020
Abstract Simple sequence repeat (SSR) enriched libraries for five groups of tropical perennial plants
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10722-020-00965-w) contains supplementary material, which is available to authorized users.
with edible fruits and shoots were prepared and sequenced in a GS-FLX Roche 454: sapodilla (Manilkara zapota (L.) P. Royen), lychee (Litchi chinensis Sonn.), mangosteen (Garcinia mangostana Linn. and G. cochinchinensis (Lour.) Choisy), rambutan (Nephelium lappaceum L.), and bamboo (Bambusa vulgaris Schrad. ex J.C. Wendl and Guadua
R. S. Arias V. A. Orner V. S. Sobolev USDA-ARS National Peanut Research Laboratory, 1011 Forrester Dr. S.E, Dawson, GA 39842, USA e-mail: [email protected]
L. L. Ballard e-mail: [email protected]
V. A. Orner e-mail: [email protected]
S. A. Simpson e-mail: [email protected]
V. S. Sobolev e-mail: [email protected]
X. F. Liu e-mail: [email protected]
R. S. Arias L. L. Ballard M. V. Duke S. A. Simpson X. F. Liu V. A. Orner V. S. Sobolev B. E. Scheffler J. Martinez-Castillo (&) USDA-ARS Plant Germplasm Introduction and Testing Research Unit, 24106 N. Bunn Rd, Prosser, WA 99350, USA e-mail: [email protected]
B. E. Scheffler e-mail: [email protected]
M. V. Duke e-mail: [email protected]
L. L. Ballard M. V. Duke S. A. Simpson X. F. Liu B. E. Scheffler USDA-ARS Genomics and Bioinformatics Research Unit, 141 Experiment Station Rd, Stoneville, MS 387761, USA
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Genet Resour Crop Evol
angustifolia Kunth). For SSR development, these species were organized by their common names in five groups. A total of 3870 SSR primer sets were designed, using capillary electrophoresis 1872 nuclear SSRs were tested on 4 to 10 DNA samples within each plant group, that is 384 loci for each of the four groups of fruit trees and 336 loci for the bamboo group. Only 7.9% of the primers tested did not result in amplification. All 1872 SSRs are provided, we highlight 178 SSRs (between 26 and 47 per group) considered topquality polymorphic SSRs that amplified all the samples, had strong fluorescence signal, presented no stutters and showed minimum non-specific amplification or background
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