Transferability of nuclear microsatellite markers to Stigmatodon species: a bromeliad genus endemic to vertical cliffs o
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GENETICS & EVOLUTIONARY BIOLOGY - SHORT COMMUNICATION
Transferability of nuclear microsatellite markers to Stigmatodon species: a bromeliad genus endemic to vertical cliffs of inselbergs in Brazil Vitor Cunha Manhães1 · Dayvid Rodrigues Couto1 · Fabiano Salgueiro2 · Andrea Ferreira da Costa3 Received: 16 June 2020 / Revised: 24 August 2020 / Accepted: 31 August 2020 © Botanical Society of Sao Paulo 2020
Abstract Stigmatodon Leme, G.K.Br. & Barfuss is one of the most representative Bromeliaceae genera that grow on the steepest slopes of the inselbergs of southeastern Brazil. Inselberg environments are characterized as islands due to their restricted, disjunct distribution and geographical isolation. These features make these sites interesting models for evolutionary and population genetic studies. In this study we report, the cross-species transferability of nuclear microsatellite markers, originally developed for other Bromeliaceae, to four Stigmatodon species: Stigmatodon brassicoides, S. costae, S. goniorachis and Stigmatodon sp. nov. Twenty-four microsatellite markers were tested, and 12 of these were successfully amplified in the four studied species. In S. brassicoides and S. costae, 10 markers showed amplification with polymorphism, and in Stigmatodon sp. nov and S. goniorachis, all 12 markers showed polymorphisms. The number of alleles ranged from two to four in S. brassicoides; from two to three in S. costae; from two to six in Stigmatodon sp. nov; and from two to seven in S. goniorachis. HO values ranged from 0.05 (P2P19 locus) in S. brassicoides to 0.689 (VgA04 locus) in S. goniorachis. The set of amplified microsatellite markers described in this study will be a useful tool for addressing various evolutionary, ecological and conservation aspects of Stigmatodon species. Keywords Atlantic Forest · Bromeliaceae · Cross-amplification · SSR · Tillandsioideae
1 Introduction Stigmatodon Leme, G.K.Br. & Barfuss belongs to the Tillandsioideae subfamily and was recently segregated from Vriesea Lindl. (Barfuss et al. 2016). Stigmatodon probably arose in the Brazilian Atlantic Forest c. 2.8–6.4 Mya (Kessous et al. 2019). Today, it is endemic to this biome and * Vitor Cunha Manhães [email protected] 1
Programa de Pós‑Graduação em Ciências Biológicas (Botânica), Universidade Federal do Rio de Janeiro, Museu Nacional, Quinta da Boa Vista, São Cristóvão, Rio de Janeiro, RJ 20949‑040, Brazil
2
Laboratório de Biodiversidade e Evolução Molecular, Departamento de Botânica, Universidade Federal do Estado do Rio de Janeiro, Av. Pasteur 458, Rio de Janeiro 22290‑255, Brazil
3
Departamento de Botânica, Museu Nacional, Universidade Federal do Rio de Janeiro, Quinta da Boa Vista, São Cristóvão, Rio de Janeiro 20949‑040, Brazil
comprises 19 species that exclusively inhabit the vertical slopes of inselbergs in the Atlantic Forest domain, southeastern Brazil (Couto and Costa 2019). Recent studies revealed four species of the genus that have very similar morphological characteristics (Couto 2017). These species
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