Developmental stage-associated microbiota profile of the peach fruit fly, Bactrocera zonata (Diptera: Tephritidae) and t
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ORIGINAL ARTICLE
Developmental stage‑associated microbiota profile of the peach fruit fly, Bactrocera zonata (Diptera: Tephritidae) and their functional prediction using 16S rRNA gene metabarcoding sequencing Naiyar Naaz1,2 · Jaipal S. Choudhary2 · Ashok Choudhary1 · Abhijit Dutta3 · Bikash Das2 Received: 7 July 2020 / Accepted: 4 August 2020 © King Abdulaziz City for Science and Technology 2020
Abstract The different developmental stage-associated microbiota of the peach fruit fly, Bactrocera zonata (Diptera: Tephritidae), was characterized using 16S rRNA gene (V3–V4 region) metabarcoding on the Illumina HiSeq platform. Taxonomically, at 97% similarity, there were total 16 bacterial phyla, comprising of 24 classes, 55 orders, 90 families and 134 genera. Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes were the most abundant phyla with Gammaproteobacteria, Alphaproteobacteria, Actinobacteria, Bacteroidia and Bacilli being the most abundant classes. The bacterial genus Enterobacter was dominant in the larval and adult stages and Pseudomonas in the pupal stage. A total of 2645 operational taxonomic units (OTUs) were identified, out of which 151 OTUs (core microbiota) were common among all the developmental stages of B. zonata. The genus Enterobacter, Klebsiella and Pantoea were dominant among the core microbiota. PICURSt analysis predicted that microbiota associated with B. zonata may be involved in membrane transport, carbohydrate metabolism, amino acid metabolism, replication and repair processes as well as in cellular processes and signalling. The microbiota that was shared by all the developmental stages of B. zonata in the present study could be targeted and the foundation for research on microbiota-based management of fruit flies. Keywords Bactrocera zonata · Ontogeny · Microbiota · 16S rRNA gene · Management
Introduction The dipteran flies of family Tephritidae are commonly known as fruit flies. The peach fruit fly, Bactrocera zonata (Saunders), is a serious insect pest of fruit crops in many parts of the world particularly in the South and South East Asia and in some parts of Africa with more than 50 plant Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13205-020-02381-4) contains supplementary material, which is available to authorized users. * Jaipal S. Choudhary [email protected] 1
University Department of Botany, Ranchi University, Morabadi, Ranchi, Jharkhand 843008, India
2
ICAR-RCER, Farming System Research Centre for Hill and Plateau Region, Plandu, Ranchi, Jharkhand 834010, India
3
University Department of Zoology, Ranchi University, Morabadi, Ranchi, Jharkhand 843008, India
species as host (Kapoor, 1993; Duyck et al. 2004; Choudhary et al. 2012; CABI/EPPO, 2013). Due to huge damage potential, B. zonata is considered as one of the most destructive insect pest species of peach, guava and mango in tropical and subtropical climatic conditions (Choudhary et al. 2012). Most of the studies related to this pest have
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