Diagnostic Virology Protocols
Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of v
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Diagnostic Virology Protocols Second Edition Edited by
John R. Stephenson Research and Development Division, Health Protection Agency, London, UK
Alan Warnes Northwick Park Hospital, Research and Development Department, North West London Hospitals NHS Trust, Harrow, Middlesex, UK
Editors Dr. John R. Stephenson Research and Development Division Health Protection Agency London UK [email protected]
Dr. Alan Warnes Northwick Park Hospital Research and Development Department North West London Hospitals NHS Trust Harrow, Middlesex UK [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-816-4 e-ISBN 978-1-60761-817-1 DOI 10.1007/978-1-60761-817-1 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010937421 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Cover illustration: See Chapter 13, Figure 1. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate, and cost-effective diagnosis of viral disease. Since the first edition of this book, there have been several major changes in the virus diagnostic laboratory. The first edition contained protocols for a number of techniques, including electron microscopy, ELISA, virus neutralisation, heamagglutinin inhibition, and electrophoresis, but nearly all the chapters in this current edition describe protocols involving nucleic acid detection, most often utilising some form of the polymerase chain reaction (PCR). We also included a chapter on th
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