Diagnostic Bacteriology Methods and Protocols
This volume provides a comprehensive collection of protocols on molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike. Chapters detail common bacterial pathogens, protoco
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Kimberly A. Bishop-Lilly Editor
Diagnostic Bacteriology Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Diagnostic Bacteriology Methods and Protocols
Edited by
Kimberly A. Bishop-Lilly Germantown, MD, USA
Editor Kimberly A. Bishop-Lilly Germantown, MD, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7035-3 ISBN 978-1-4939-7037-7 (eBook) DOI 10.1007/978-1-4939-7037-7 Library of Congress Control Number: 2017939131 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover illustration: Cover image credit to Heidi Bishop. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface This volume in the Methods in Molecular Biology series is a comprehensive collection of protocols in molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike. The benefits of applying a molecular diagnostic approach for bacterial detection and identification are many—including reduced time (especially for difficult to culture or slow-growing organisms) and, in some cases, higher specificity (such as strain-level identification rather than species-level identification). In the case of high-throughput sequencing, one may even collect all the information one needs to know about a pathogen (such as genus-, species-, and strain-level identification along with virulence potential and antibiotic resistance potential) from a single reaction, rather tha
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