Diamond-like Carbon Film Coating for Tissue Engineering
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Diamond-like Carbon Film Coating for Tissue Engineering Yasuharu Ohgoe1, Haruki Matsuo1, Kazuhiro Nonaka1, Toshiyuki Yaguchi1, Kazuya Kanasugi1, Kenji K. Hirakuri2, Akio Funakubo1, and Yasuhiro Fukui1 1 Tokyo Denki University, Ishizaka, Hatoyama, Saitama 350-0394, Japan 2 Tokyo Denki University, 2-2 Kanda-Nishiki-cho, Chiyoda-ku, Tokyo 101-8457, Japan ABSTRACT In this study, we focus on effect of diamond-like carbon (DLC) coating on scaffold for tissue engineering. DLC film was deposited on segmented polyurethane (SPU) scaffold sheet which consists of micro SUP fibers. Structural and compositional effects of the DLC film coating were investigated on cell growth as an investigation of biological response. The surface composition, morphology, structures, and wettability of the DLC film coating was estimated by using X-ray photoelectron spectrometer (XPS), Scanning Electron Microscope (SEM), Ar-laser Raman spectrophotometer (Raman), and contact angle measurement. And then, human umbilical vein endothelial (HUV-EC-C) cells were grown on the DLC coated scaffold sheet. The results presented here suggest that DLC film coating is promising approach to improve biological for tissue engineering. INTRODUCTION A synthetic vascular graft has already been used for clinical surgery. One major problem that all cardiovascular replacements have in common is lack of cytocompatibility for endothelial cell growth [1]. The lack of cytocompatibility causes substantial risk of thrombosis problems during long implantation periods. In order to minimize or avoid the risks, tissue engineering, whereby living tissue replacements can be constructed, has emerged as a solution to some of the problems. The emerging field of tissue engineering focuses on the in vitro generation of functional organ replacements using living endothelial cells and other cells for which nondegradable or biodegradable scaffold base materials are used [1]. This approach has the potential for improving biocompatibility of various types of implant appliances including vascular grafts. However, it was indicated that several in vitro studies have shown most of the metallic and polymeric materials do not support endothelial cell growth [1]. In recent studies in tissue engineering, use of polyurethanes in tissue-organ regeneration scaffolds for replacement or repair of a wide range of biological tissues is an area of intensive research [2]. Efficient attachment, survival and release of biologically active agents by endothelial cells are serious dependent on characteristics of scaffold. Therefore, improvement method of surface modification for scaffold is very important factor in this field. In our previous works, we deposited diamond-like carbon (DLC) film on inner-wall of a synthetic vascular graft (expanded polytetrafluoroethylene: ePTFE), and successfully improved cell growth (mouse fibroblasts: NIH 3T3). As well known, DLC film has attractive properties of biocompatibility including blood compatibility and cytocompatibility. Furthermore, DLC films are also con
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