Different resistance potential to reovirus in grass carp ( Ctenopharyngodon idella ) populations and their immune charac
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Different resistance potential to reovirus in grass carp (Ctenopharyngodon idella) populations and their immune characteristics Hongquan Wang 1
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& Hong Yang & Rui Li & Yaoguo Li & Tiaoyi Xiao
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Received: 29 August 2020 / Accepted: 17 November 2020/ # Springer Nature Switzerland AG 2020
Abstract
Our aim was to verify whether genes in the coagulation complement and cytokine interaction pathways are crucial to the resistance to grass carp hemorrhagic disease in grass carps (Ctenopharyngodon idella). To evaluate this, we acquired male grass carps with grass carp reovirus (GCRV) resistance, F1 generation, ordinary male fish, control F1 progenies (hybridized ordinary fish), and barbel chub (Squaliobarbus curriculus) as a positive control. We compared the expression variation of 13 immune factors in different tissues using real-time fluorescent quantitative PCR. Our results showed that the expression of the transforming growth factor-β3, nerve growth factor receptor, coagulation factor D, chemokine receptor XCR1, profibrinolysin, γ-globulin of complement component 1, complement component 2, and complement component 5 in the different tissues of the barbel chub were significantly higher than those in the grass carps populations under normal conditions. Except for a limited number of genes, their expression exhibited significant differences. There was no significant difference in these genes among grass carp populations with different GCRV resistance. These results indicate that the high gene expression in the coagulation complement and cytokine interaction pathways may be the main reason for the strong resistance of barbel chub to GCRV. However, high gene expression was not a factor affecting GCRV resistance between grass carp populations. Keywords Virus resistance . Carp . Population variation . Gene expression . Grass carp reovirus . Hemorrhagic disease
* Tiaoyi Xiao [email protected]
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College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
Aquaculture International
Introduction Grass carp (Ctenopharyngodon idella) is one of the most important freshwater cultured fish species in China (Ni et al. 2014). In 2018, grass carp production in China reached 5.50 million tonnes, accounting for 21.63% of the total production of all freshwater cultured fish in China (Fish and Fisheries Administration of Ministry of Agriculture and Rural Affairs of the People’s Republic of China et al. 2019). However, this industry has been seriously hampered by a severe hemorrhagic disease caused by grass carp reovirus (GCRV) (Zhang et al. 2007; Wang et al. 2019). Infection with GCRV causes a mortality rate of up to 85% during the summer months (Liang et al. 2014). GCRV results in at least one billion US dollars in annual economic losses in China (Zeng et al. 2014). Stress selection breeding, cross breeding, cell engineering (Li and Mao 1990), gene engineering (Zhu et al. 1985; Zhang et al. 1997), and other methods are usually used in stress resistance breeding. In the breeding of GCRV-re
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