Differential expression profile of microRNA in yak skeletal muscle and adipose tissue during development
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Genes & Genomics https://doi.org/10.1007/s13258-020-00988-8
RESEARCH ARTICLE
Differential expression profile of microRNA in yak skeletal muscle and adipose tissue during development Hui Ji1 · Hui Wang1 · Qiumei Ji2 · Winbo Ji1 · Xiaolin Luo3 · Jiabo Wang1 · Zhixin Chai1 · Jinwei Xin2 · Xin Cai1 · Zhijuan Wu1 · Jikun Wang1 · Jincheng Zhong1 Received: 11 May 2020 / Accepted: 13 August 2020 © The Genetics Society of Korea 2020
Abstract Background miRNAs play an important role in regulating normal animal development. Muscle tissue and fat metabolism are important for maintaining energy balance in animals. Yak has important agricultural and economic importance as it provides milk, meat, and hair. It is used for transportation as well. However, the miRNA expression profiles of their muscle and adipose tissue are currently unknown. Objective To explore the regulatory roles of miRNAs in the skeletal muscle and adipose tissues of yak. Methods A total of 12 small RNA libraries were constructed from the skeletal muscle and adipose samples from yak aged 0.5, 2.5, 4.5, and 7.5 years. High-throughput sequencing and bioinformatics analysis were used to determine the dynamic expression profile of miRNA, and a miRNA regulatory network related to muscle and adipose tissue development was established. Results miR-1-3p and miR-143-3p showed the highest expression during yak skeletal muscle and fat development, respectively. The MAPK and Ras signaling pathways were the pivotal pathways. miR-181-5p, miR-542-3p, and miR-424-5p may have key roles in skeletal muscle development, and CREBRF, GRB10, CDK1, RFX3, and EPC2 were the core target genes. While miR-127-5p, miR-379-3p, and miR-494-3p may play important regulatory roles in adipose deposition, and ETV1, XPO7, and C5AR2 were the core target genes. Conclusion This study provides valuable resources for further study of the molecular mechanisms underlying yak skeletal muscle and adipose tissue development, and also a basis for studying the interactions between genes and miRNAs. Keywords Yak · Skeletal muscle · Adipose · miRNA Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13258-020-00988-8) contains supplementary material, which is available to authorized users. * Jincheng Zhong [email protected]
Jinwei Xin [email protected]
Hui Ji [email protected]
Xin Cai [email protected]
Hui Wang [email protected] Qiumei Ji [email protected] Winbo Ji [email protected]
Zhijuan Wu [email protected] Jikun Wang [email protected] 1
Xiaolin Luo [email protected]
Southwest Minzu University, No. 16, South Fourth Section, First Ring Road, Chengdu City 610041, Sichuan Province, China
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Jiabo Wang [email protected]
Tibet Academy of Agriculture and Animal Husbandry, No. 130 Jinzhu West Road, Lhasa 850000, Tibet, China
3
Sichuan Grassland Science Academy, No. 368 Guoning West Road, Pidu District, Chengdu City 611731, Sichuan Province, China
Zhixin Chai [email protected]
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