Diosgenin attenuates inflammatory response induced by myocardial reperfusion injury: role of mitochondrial ATP-sensitive
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ORIGINAL PAPER
Diosgenin attenuates inflammatory response induced by myocardial reperfusion injury: role of mitochondrial ATP-sensitive potassium channels Hadi Ebrahimi & Reza Badalzadeh & Mustafa Mohammadi & Bahman Yousefi
Received: 3 August 2013 / Accepted: 3 February 2014 # University of Navarra 2014
Abstract Reperfusion injury is one of the main reasons of cardiac disease morbidity. Phytopharmaceuticals are gaining importance in m odern medicine of cardioprotection because of their multiplex capacity. The aim of this study was to investigate the effect of diosgenin on the inflammatory response induced by myocardial ischemia and reperfusion injury and the role of mitochondrial ATP-sensitive potassium (mitoKATP) channels in this regard. Wistar rats (250–300 g) were used in this study. The Langendorff-perfused hearts of animals were subjected to a 30-min global ischemia followed by a 90-min reperfusion. The lactate dehydrogenase (LDH) release was measured by spectrophotometry. The levels of inflammatory mediators tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and IL-6 in the supernatant of heart’s left ventricle were measured using an enzyme-linked immunosorbent assay rat specific ELISA kit. The LDH release into the coronary effluent during reperfusion was significantly
H. Ebrahimi : R. Badalzadeh Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran R. Badalzadeh (*) : M. Mohammadi Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran e-mail: [email protected] B. Yousefi Department of Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
decreased, and cardiac contractility significantly improved by diosgenin preadministration as compared with those of control or Cremophor-EL (solvent of diosgenin) groups (398±48 vs. 665±65 or 650±73 ml/ min) (P
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