Soshiho-tang protects LPS-induced acute liver injury by attenuating inflammatory response
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Soshiho‑tang protects LPS‑induced acute liver injury by attenuating inflammatory response Seong Chul Jin1 · Mi Hye Kim1 · Si Yeon Jo1 · La Yoon Choi1 · Haesu Lee1 · Woong Mo Yang1 Received: 6 September 2019 / Accepted: 3 June 2020 © The Japanese Society of Pharmacognosy 2020
Abstract Soshiho-tang (SSHT) has traditionally been used to treat gastrointestinal disorders. In this experiment, we investigated the protective effect of SSHT on inflammatory liver injury in lipopolysaccharide (LPS)-sensitized mice. Male C57BL/6J mice aged 6 weeks were randomly placed in 6 groups (n = 5): normal mice (CTR), LPS-sensitized mice (LPS), LPS-sensitized mice treated with dexamethasone (DEX) and LPS-sensitized mice treated with 0.05, 0.55, and 5.55 g/kg of SSHT (SSHT 0.05, SSHT 0.55, and SSHT 5.55). Various doses of SSHT was given once a day for 7 days. After 2 h of LPS injection, the liver tissue was collected. SSHT pretreatment recovered hemorrhage of liver tissues in LPS-induced acute liver injury. The expressions of MAP Kinase, NF-κB, IκBα, p-IκBα, COX-2, and iNOS protein levels were markedly decreased by SSHTtreated liver tissues. Additionally, SSHT pretreatment significantly regulated the expressions of MCP-1, TNF-α, and IL-6 cytokines. These results suggest the potential of SSHT on the protection of acute liver injury. Keywords Soshiho-tang · Liver inflammation · LPS · Acute liver injury · Anti-inflammation
Introduction The role of the liver is metabolism, detoxification, immune function, and bile secretion of major nutrients [1]. Approximately 2 million per year suffers from liver disease and 1 million has complications such as cirrhosis, viral hepatitis, and hepatocellular carcinoma [2]. Liver disease is caused by a variety of factors including gene, viruses, alcohol, obesity, and endotoxin [3]. Inflammatory liver injury triggers liver disease by damage of hepatic tissue [4]. Treatments for acute liver injury are non-steroidal anti-inflammatory drugs such as salicylates, propionic acid, oxicam, and fenamate; however, those medications are reported to induce renal toxicity and gastrointestinal problems [5]. Sosiho-tang (SSHT) has been used for anorexia, nausea and vomiting as one of the representative traditional prescriptions in East Asia [6]. The components of SSHT are seven kinds of herbal medicines including Bupleurum falcatum, Scutellaria baicalensis, Panax ginseng, Pinellia ternate, * Woong Mo Yang [email protected] 1
Department of Convergence Korean Medical Science, Graduate School, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea
Glycyrrhiza uralensis, Zingiber officinale and Ziziphus jujube. SSHT has several pharmacological effects such as immune modulating [7], liver regeneration [8], anti-inflammatory [9], antioxidant, and anti-tumor effects [10]. Exposure to excessive dose of lipopolysaccharides (LPS) causes the impairment of liver function and hepatocyte degeneration by inducing inflammatory responses [11]. In LPSinduced acute liver injury, pro-inflammatory cytokines
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