Dispersive Raman Analysis of Sacha Inchi Ozonated Oil
The sacha inchi oil has major advantages when compared to other vegetable oils used in the pharmaceutical, food and cosmetics due to its high concentration of organic compounds. Raman spectroscopy is an analytical technique that allows real-time analysis
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UNICASTELO – Universidade Camilo Castelo Branco, R. Carolina Fonseca, 235, Itaquera, CEP 08230-030, São Paulo, SP, Brasil. CITÉ – Associação Cidade da Ciência Tecnologia e Educação, Estrada Dr. Altino Bondesan, 500, Eugênio de Melo, CEP 12247-016, São José dos Campos, SP, Brasil. 3 UAM – Universidade Anhembi Morumbi, Rua Casa do Ator, 294, 7º. andar, Unidade 5, Campus Vila Olímpia, Itaim Bibi, CEP 04546-001, São Paulo, SP, Brasil.
Abstract— The sacha inchi oil has major advantages when compared to other vegetable oils used in the pharmaceutical, food and cosmetics due to its high concentration of organic compounds. Raman spectroscopy is an analytical technique that allows real-time analysis and nondestructive way the physical-chemical composition of vegetable oils. In this work the Raman spectrum of sacha inchi oil ozonated was characterized by correlating the respective functional groups by PCA. Knowledge of the spectral changes of sacha inchi ozonated oil is important to study and obtain new therapeutic processes.
troscopy technique, which allows to evaluate the spectral components of the constituents of vegetable oils [8-11]. This study aims to evaluate the composition of ozonated sacha inchi oil, aiming future applications in ozone therapy.
Keywords— ozone, sacha inchi oil, Raman spectroscopy.
The sacha inchi oil (P. volubilis L.), obtained by pressing of dried seeds, was donated by farmers in Antioquia region (Colombia). The ozonation was performed using 10 ml of weeding sacha inchi oil, placed in a beaker using a stainless steel diffuser attached to a silicone tube with an internal diameter of 4.5 mm. The hose was connected to the output of an ozone generator (OzonLife, Medical Systems, Brazil) set to generate 66 mg/l of ozone, oxygen flow ˡ l/min. The experiment was carried out with the aid of a spectrometer Ra-man dispersive (Raman Dimension P-1, Lambda Solutions, USA), operating in the near infrared, with 830 nm excitation wavelength, optical power of 230 mW, resolution 2 spectrograph cm-1, and spectral band range of 600-1800 cm-1. The exposure time for obtaining the spectra was 1 second and 10 accumulations, totalizing 10 sec. These parameters were defined based on previous work by our group considering the optical scattering characteristics of the oil. The spectral analysis was obtained from the average of three signals collected from each of the ozonated oil samples. Of each sample were extracted three aliquots of 50 μl, they were placed in three different wells of a sample holder of aluminum. The spectrum of sample from each well was collected in five conditions: 0, 5, 10, 20 and 40 minutes of ozonation, with the distal Raman probe positioned 1 cm from the sample surface. To ensure the reproducibility of
I. INTRODUCTION
The sacha inchi (Plukenetia volubilis L.) oleaginous plant originated in the Amazon forest is rich in protein and vegetable oil [1, 2]. The oil is extracted from the pressing of their minds up, it is mainly characterized by a high content of polyunsaturated fatty acids (omega 3, 6
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